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A-RAF Antibody (ARAF1) (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7809c | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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A-RAF Antibody (ARAF1) (Center) - Product info | |
| Application | WB, IHC
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| Primary Accession | P10398 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 67585 Da |
A-RAF Antibody (ARAF1) (Center) - Additional info | |
| Gene ID 369 | |
| Other Names ARAF; ARAF1; PKS; PKS2; Serine/threonine-protein kinase A-Raf; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks | |
| Target/Specificity This A-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263~292 amino acids from the center region of human ARAF1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions A-RAF Antibody (ARAF1) (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
A-RAF Antibody (ARAF1) (Center) - Protein Information | |
| Name ARAF | |
| Synonyms ARAF1, PKS, PKS2 | |
| Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus | |
| Tissue Location Predominantly in urogenital tissues. | |
A-RAF Antibody (ARAF1) (Center) - Related products
A-RAF Antibody (ARAF1) (Center) - Application data
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Western blot analysis of hARAF1-H270 (Cat. #AP7809c) in MCF7 cell line lysates (35ug/lane). ARAF1 (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
A-RAF Antibody (ARAF1) (Center) - Research Areas
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BACKGROUND
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
REFERENCES
Wu, X., et al., J. Biol. Chem. 271(6):3265-3271 (1996). Lee, J.E., et al., Genomics 20(1):43-55 (1994). Popescu, N.C., et al., Oncogene 4(4):517-519 (1989). Beck, T.W., et al., Nucleic Acids Res. 15(2):595-609 (1987). Yin, X.L., et al., Mol. Cell. Biochem. 231 (1-2), 69-74 (2002).
