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B-RAF Antibody (T439)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7810i | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
B-RAF Antibody (T439) - Product info | |
| Application | WB, IHC
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| Primary Accession | P15056 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 84437 Da |
B-RAF Antibody (T439) - Additional info | |
| Gene ID 673 | |
| Other Names BRAF; BRAF1; RAFB1; Serine/threonine-protein kinase B-raf; Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 | |
| Target/Specificity This B-RAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T439 of human BRAF. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions B-RAF Antibody (T439) is for research use only and not for use in diagnostic or therapeutic procedures. | |
B-RAF Antibody (T439) - Protein Information | |
| Name BRAF | |
| Synonyms BRAF1, RAFB1 | |
| Function Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron | |
| Cellular Location Nucleus (By similarity). Cytoplasm. Cell membrane (By similarity). Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes (By similarity) | |
| Tissue Location Brain and testis. | |
B-RAF Antibody (T439) - Related products
AP12058c: BRAF Antibody (Center S364)
AP3303a: Phospho-BRAF-T439 Antibody
AP3373a: Phospho-BRAF-S601 Antibody
AP3406a: Phospho-BRAF-pT598 Antibody
AP3418a: Phospho-BRAF-pS445 Antibody
AP3513a: Phospho-BRAF-pT400 Antibody
AP7810c: B-RAF Antibody (Center)
AP7810d: B-RAF Antibody (T372)
AP7810f: B-RAF Antibody (S445)
AP7810h: B-RAF Antibody (S728)
AP7810i: B-RAF Antibody (T439)
AP7810j: B-RAF Antibody (S601)
AP7810k: RAF1 (BRAF) Antibody (T598)
AP7810l: B-RAF Antibody (S428)
AP7810m: B-RAF Antibody (S364)
BP3303a: Phospho-BRAF-T439 Antibody Blocking Peptide
BP3373a: Phospho-BRAF-S601 Antibody Blocking Peptide
BP3406a: Phospho-BRAF-pT598 Antibody Blocking Peptide
BP3418a: Phospho-BRAF-pS445 Antibody Blocking Peptide
BP3513a: Phospho-BRAF-pT400 Antibody Blocking Peptide
BP7810c: BRAF Antibody (Center) Blocking Peptide
BP7810d: BRAF Antibody (T372) Blocking Peptide
BP7810e: BRAF Antibody (T400) Blocking Peptide
BP7810f: BRAF Antibody (S445) Blocking Peptide
BP7810g: BRAF Antibody (S578) Blocking Peptide
BP7810h: BRAF Antibody (S728) Blocking Peptide
BP7810i: BRAF Antibody (T439) Blocking Peptide
BP7810j: BRAF Antibody (S601) Blocking Peptide
BP7810k: BRAF Antibody (T598) Blocking Peptide
B-RAF Antibody (T439) - Application data
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Western blot analysis of BRAF (arrow) using rabbit polyclonal BRAF Antibody (T439) (RB10937). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BRAF gene (Lane 2) (Origene Technologies).
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Formalin-fixed and paraffin-embedded human brain tissue reacted with BRAF Antibody (T439) (Cat.#AP7810i), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
B-RAF Antibody (T439) - Research Areas
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BACKGROUND
BRAF, a member of the RAF subfamily of Ser/Thr protein kinases, is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neurons. This cytoplasmic protein is expressed in brain and testis. Defects in BRAF are involved in a wide range of cancers including lung cancer and non-Hodgkin lymphoma (NHL). This protein contains 1 zinc-dependent phorbol-ester and DAG binding domain.
REFERENCES
Hingorani, S.R., et al., Cancer Res. 63(17):5198-5202 (2003). Lee, J.W., et al., Br. J. Cancer 89(10):1958-1960 (2003). Davies, H., et al., Nature 417(6892):949-954 (2002). Naoki, K., et al., Cancer Res. 62(23):7001-7003 (2002). Stephens, R.M., et al., Mol. Cell. Biol. 12(9):3733-3742 (1992).
