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Transglutaminase (TGM2) Antibody (Center K444)

Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - Transglutaminase (TGM2) Antibody (Center K444) AP7826d
    Anti-TGM2 Antibody (Center K444) at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Transglutaminase (TGM2) Antibody (Center K444) AP7826d
    TGM2 Antibody (Center K444) (Cat. #AP7826d) western blot analysis in HUVEC,K562 cell line lysates (35ug/lane).This demonstrates the TGM2 antibody detected the TGM2 protein (arrow).
  • IF - Transglutaminase (TGM2) Antibody (Center K444) AP7826d
    Fluorescent confocal image of U251 cell stained with TGM2 Antibody (Center K444)(Cat#AP7826d).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TGM2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TGM2 immunoreactivity is localized to Cytoplasm significantly.
  • IHC-P - Transglutaminase (TGM2) Antibody (Center K444) AP7826d
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with *TGM2 antibody (Center K444), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P21980
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 77329 Da
Antigen Region 429-458 aa
Additional Information
Gene ID 7052
Other Names Protein-glutamine gamma-glutamyltransferase 2, Tissue transglutaminase, Transglutaminase C, TG(C), TGC, TGase C, Transglutaminase H, TGase H, Transglutaminase-2, TGase-2, TGM2
Target/Specificity This Transglutaminase (TGM2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 429-458 amino acids from the Central region of human Transglutaminase (TGM2).
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTransglutaminase (TGM2) Antibody (Center K444) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TGM2
Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Research Areas
Citations (0)

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Transglutaminases are enzymes that catalyze the crosslinking of proteins by epsilon-gamma glutamyl lysine isopeptide bonds. While the primary structure of transglutaminases is not conserved, they all have the same amino acid sequence at their active sites and their activity is calcium-dependent. TGM2 acts as a monomer, is induced by retinoic acid, and appears to be involved in apoptosis. It has been identified as the autoantigen implicated in celiac disease.


Porzio,O., Hum. Mutat. 28 (11), 1150 (2007)
Parsons,A.C., Am J Dermatopathol 29 (5), 433-436 (2007)

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$ 295.00
$ 99.00
Cat# AP7826d
(40 western blots)
Availability: In Stock
Bulk Size
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