|Application ||WB, IHC-P, E|
|Calculated MW||66591 Da|
|Antigen Region||564-593 aa|
|Other Names||Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2|
|Target/Specificity||This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Methionyl tRNA Synthetase Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Mitochondrion matrix.|
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Provided below are standard protocols that you may find useful for product applications.
Methionine-tRNA ligase (EC 220.127.116.11) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met). This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.
Spencer,A.C., Biochemistry 43 (30), 9743-9754 (2004)
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