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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Methionyl tRNA Synthetase Antibody (C-term)   

Methionyl tRNA Synthetase Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Methionyl tRNA Synthetase Antibody (C-term) AP7841b
    Anti-MARS2 Antibody (C-term) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Methionyl tRNA Synthetase Antibody (C-term) AP7841b
    Western blot analysis of anti-MARS2 Antibody (C-term) (AP7841b) in mouse testis tissue lysates (35ug/lane). MARS2(arrow) was detected using the purified Pab.
  • IHC-P - Methionyl tRNA Synthetase Antibody (C-term) AP7841b
    Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with MARS2 antibody (C-term) (Cat.#AP7841b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q96GW9
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 66591 Da
Antigen Region 564-593 aa
Additional Information
Gene ID 92935
Other Names Methionine--tRNA ligase, mitochondrial, Methionyl-tRNA synthetase 2, Mitochondrial methionyl-tRNA synthetase, MtMetRS, MARS2
Target/Specificity This Methionyl tRNA Synthetase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 564-593 amino acids from the C-terminal region of human Methionyl tRNA Synthetase.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMethionyl tRNA Synthetase Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MARS2
Cellular Location Mitochondrion matrix.
Research Areas
Citations (0)

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Background

Methionine-tRNA ligase (EC 6.1.1.10) catalyzes the chemical reaction: ATP + L-methionine + tRNAMet <--> AMP + diphosphate + L-methionyl-tRNAMet The 3 substrates of this enzyme are ATP, L-methionine, and tRNA(Met), whereas its 3 products are AMP, diphosphate, and L-methionyl-tRNA(Met). This enzyme participates in 3 metabolic pathways: methionine metabolism, selenoamino acid metabolism, and aminoacyl-trna biosynthesis.

References

Spencer,A.C., Biochemistry 43 (30), 9743-9754 (2004)

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$ 295.00
$ 99.00
Cat# AP7841b
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(40 western blots)
Availability: In Stock
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