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VARS Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - VARS Antibody (N-term) AP7843a
    Western blot analysis of anti-VARS Antibody (N-term) (Cat.#AP7843a) in K562 cell line lysates (35ug/lane).VARS (arrow) was detected using the purified Pab.
    detail
  • IHC-P - VARS Antibody (N-term) AP7843a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with the VARS antibody (N-term) (Cat.#AP7834a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P26640
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 140476 Da
Antigen Region 251-279 aa
Additional Information
Gene ID 7407
Other Names Valine--tRNA ligase, Protein G7a, Valyl-tRNA synthetase, ValRS, VARS, G7A, VARS2
Target/Specificity This VARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-279 amino acids from the N-terminal region of human VARS.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsVARS Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name VARS1 (HGNC:12651)
Synonyms G7A, VARS, VARS2
Function Catalyzes the attachment of valine to tRNA(Val).
Citations (0)
citation

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Background

Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. This protein belongs to the class-I aminoacyl-tRNA synthetase family and is located in the class III region of the major histocompatibility complex.

References

Jiang,S., FEBS Lett. 579 (27), 6049-6054 (2005)
Bonnefond,L., Biochemistry 44 (12), 4805-4816 (2005)
Xie,T., Genome Res. 13 (12), 2621-2636 (2003)

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$ 182.50
$ 70.00
Cat# AP7843a
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