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ALDH9A1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - ALDH9A1 Antibody (N-term) AP7850a
    Western blot analysis of anti-ALDH9A1 Antibody (N-term) (Cat.#AP7850a) in mouse kidney tissue lysates (35ug/lane). ALDH9A1(arrow) was detected using the purified Pab.
    detail
  • IHC-P - ALDH9A1 Antibody (N-term) AP7850a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ALDH9A1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession P49189
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 53802 Da
Antigen Region 4-34 aa
Additional Information
Gene ID 223
Other Names 4-trimethylaminobutyraldehyde dehydrogenase, TMABADH, Aldehyde dehydrogenase E3 isozyme, Aldehyde dehydrogenase family 9 member A1, Gamma-aminobutyraldehyde dehydrogenase, R-aminobutyraldehyde dehydrogenase, ALDH9A1, ALDH4, ALDH7, ALDH9
Target/Specificity This ALDH9A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human ALDH9A1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsALDH9A1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ALDH9A1
Synonyms ALDH4, ALDH7, ALDH9 {ECO:0000303|PubMed:
Function Converts gamma-trimethylaminobutyraldehyde into gamma- butyrobetaine with high efficiency (in vitro). Can catalyze the irreversible oxidation of a broad range of aldehydes to the corresponding acids in an NAD-dependent reaction, but with low efficiency. Catalyzes the oxidation of aldehydes arising from biogenic amines and polyamines.
Cellular Location Cytoplasm, cytosol {ECO:0000250|UniProtKB:Q9JLJ3}. Cytoplasm
Tissue Location Detected in brain (at protein level) (PubMed:8645224). High expression in adult liver, skeletal muscle, and kidney. Low levels in heart, pancreas, lung and brain (PubMed:8786138) Expressed in all regions of the brain. Expression levels are variable in the different brain areas, with the highest levels in the spinal cord and the lowest in the occipital pole.
Research Areas
Citations ( 0 )

Background

ALDH9A1 belongs to the aldehyde dehydrogenase family of proteins. The protein has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits.

References

Cheung,C.L., Hum. Mol. Genet. 18 (4), 679-687 (2009)
Vaz,F.M., J. Biol. Chem. 275 (10), 7390-7394 (2000)
Lin,S.W., Genomics 34 (3), 376-380 (1996)

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$ 182.50
$ 70.00
Cat# AP7850a
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