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ACHE Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP7853a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
ACHE Antibody (N-term) - Product info | |
| Application | WB, IHC, FC, IF
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| Primary Accession | P22303 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 67796 Da |
ACHE Antibody (N-term) - Additional info | |
| Gene ID 43 | |
| Other Names ACHE; Acetylcholinesterase | |
| Target/Specificity This ACHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 154~183 amino acids from the N-terminal region of human ACHE. | |
| Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50 FC~~1:10~50 IF~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions ACHE Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
ACHE Antibody (N-term) - Protein Information | |
| Name ACHE | |
| Function Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis | |
| Cellular Location Cell junction, synapse. Secreted (By similarity). Cell membrane; Peripheral membrane protein (By similarity) Isoform H: Cell membrane; Lipid-anchor, GPI- anchor; Extracellular side (By similarity) | |
| Tissue Location Isoform H is highly expressed in erythrocytes. | |
ACHE Antibody (N-term) - Related products
AM2184a: ACHE Antibody (C-term) (Ascites)
AP7853a: ACHE Antibody (N-term)
AP7853b: ACHE Antibody (C-term)
BP7853a: ACHE Antibody (N-term) Blocking Peptide
BP7853b: ACHE Antibody (C-term) Blocking Peptide
ACHE Antibody (N-term) - Application data
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Western blot analysis of ACHE Antibody (N-term) (Cat.#AP7853a) in Jurkat, Y79 and NCI-H460 cell line lysates (35ug/lane). ACHE (arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ACHE antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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ACHE Antibody (N-term) (Cat. #AP7853a) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Confocal immunofluorescent analysis of ACHE Antibody (N-term)(Cat#AP7853a) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
ACHE Antibody (N-term) - Research Areas
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BACKGROUND
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
REFERENCES
Liang,D., FEBS J. 276 (1), 94-108 (2009) Scacchi,R., Am. J. Med. Genet. B Neuropsychiatr. Genet. (2008)
