|Application ||WB, E|
|Other Accession||F1SPM8, F1MH24|
|Calculated MW||103885 Da|
|Antigen Region||186-216 aa|
|Other Names||AP2-associated protein kinase 1, Adaptor-associated kinase 1, AAK1, KIAA1048|
|Target/Specificity||This AAK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the N-terminal region of human AAK1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||AAK1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates clathrin-mediated endocytosis by phosphorylating the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2) which ensures high affinity binding of AP-2 to cargo membrane proteins during the initial stages of endocytosis. Isoform 1 and isoform 2 display similar levels of kinase activity towards AP2M1. Regulates phosphorylation of other AP-2 subunits as well as AP-2 localization and AP-2-mediated internalization of ligand complexes. Phosphorylates NUMB and regulates its cellular localization, promoting NUMB localization to endosomes. Binds to and stabilizes the activated form of NOTCH1, increases its localization in endosomes and regulates its transcriptional activity.|
|Cellular Location||Cell membrane; Peripheral membrane protein. Membrane, clathrin-coated pit. Note=Active when found in clathrin-coated pits at the plasma membrane. In neuronal cells, enriched at presynaptic terminals. In non-neuronal cells, enriched at leading edge of migrating cells (By similarity).|
|Tissue Location||Detected in brain, heart and liver. Isoform 1 is the predominant isoform in brain|
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Provided below are standard protocols that you may find useful for product applications.
Adaptor-related protein complex 2 (AP-2 complexes) functions during receptor-mediated endocytosis to trigger clathrin assembly, interact with membrane-bound receptors, and recruit encodytic accessory factors. AAK1 is a member of the SNF1 subfamily of Ser/Thr protein kinases. The protein interacts with and phosphorylates a subunit of the AP-2 complex, which promotes binding of AP-2 to sorting signals found in membrane-bound receptors and subsequent receptor endocytosis. Its kinase activity is stimulated by clathrin.
Henderson,D.M., Mol. Biol. Cell 18 (7), 2698-2706 (2007)
Takahashi,T., Cancer Res. 66 (24), 11932-11937 (2006)
Schmid,E.M., PLoS Biol. 4 (9), E262 (2006)
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