- CITATIONS: 1
|Application ||WB, IHC-P, IF, FC, E|
|Calculated MW||55887 Da|
|Antigen Region||216-246 aa|
|Other Names||Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B, CYP21A2, CYP21, CYP21B|
|Target/Specificity||This CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 216-246 amino acids from the Central region of human CYP21A2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CYP21A2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids (PubMed:22014889).|
|Cellular Location||Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein|
Provided below are standard protocols that you may find useful for product applications.
CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.
Guerra-Junior,G., Clin. Exp. Immunol. 155 (2), 182-188 (2009) Abid,F., Clin. Chem. Lab. Med. 46 (12), 1707-1713 (2008) Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)
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