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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   CYP2F1 Antibody (C-term)   

CYP2F1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - CYP2F1 Antibody (C-term) AP7883b
    Western blot analysis of CYP2F1 Antibody (C-term) (Cat. #AP7883b) in A2058 cell line lysates (35ug/lane).CYP2F1 (arrow) was detected using the purified Pab.
  • IHC-P - CYP2F1 Antibody (C-term) AP7883b
    Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CYP2F1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P24903
Other Accession O35293
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 55501 Da
Antigen Region 395-424 aa
Additional Information
Gene ID 1572
Other Names Cytochrome P450 2F1, CYPIIF1, CYP2F1
Target/Specificity This CYP2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-424 amino acids from the C-terminal region of human CYP2F1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCYP2F1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CYP2F1
Function May be involved in the metabolism of various pneumotoxicants including naphthalene. Is able to dealkylate ethoxycoumarin, propoxycoumarin, and pentoxyresorufin but possesses no activity toward ethoxyresorufin and only trace dearylation activity toward benzyloxyresorufin. Bioactivates 3- methylindole (3MI) by dehydrogenation to the putative electrophile 3-methylene-indolenine.
Cellular Location Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein
Tissue Location Expressed in lung. Rarely detected in liver and placenta.
Research Areas
Citations (0)

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CYP2F1 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to dehydrogenate 3-methylindole, an endogenous toxin derived from the fermentation of tryptophan, as well as xenobiotic substrates such as naphthalene and ethoxycoumarin.


Tournel,G., Xenobiotica 37 (12), 1433-1438 (2007)
Tournel,G., Mutat. Res. 617 (1-2), 79-89 (2007)
Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)

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Cat# AP7883b
(40 western blots)
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