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CYP4F12 Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, E |
|---|---|
| Primary Accession | Q9HCS2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60309 Da |
| Antigen Region | 473-503 aa |
| Gene ID | 66002 |
|---|---|
| Other Names | Cytochrome P450 4F12, CYPIVF12, CYP4F12 |
| Target/Specificity | This CYP4F12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-503 amino acids from the C-terminal region of human CYP4F12. |
| Dilution | WB~~1:1000 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | CYP4F12 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CYP4F12 {ECO:0000303|PubMed:16112640, ECO:0000312|HGNC:HGNC:18857} |
|---|---|
| Function | A cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds, with preference for omega-2 position. Metabolizes (5Z,8Z,11Z,14Z)- eicosatetraenoic acid (arachidonate) toward 18-hydroxy arachidonate (PubMed:11162607). Catalyzes the epoxidation of double bonds of PUFAs such as docosapentaenoic and docosahexaenoic acids (PubMed:16112640). Has low omega-hydroxylase activity toward leukotriene B4 and arachidonate (PubMed:11162645). Involved in the metabolism of xenobiotics. Catalyzes the hydroxylation of the antihistamine drug ebastine (PubMed:11162645). |
| Cellular Location | Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9HBI6}. Microsome membrane {ECO:0000250|UniProtKB:Q9HBI6} |
| Tissue Location | Expressed in small intestine, liver, colon and heart. |
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Provided below are standard protocols that you may find useful for product applications.
Background
CYP4F12 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein likely localizes to the endoplasmic reticulum.
References
Dhar,M., J. Lipid Res. 49 (3), 612-624 (2008)
Stark,K., Arch. Biochem. Biophys. 441 (2), 174-181 (2005)
Nelson,D.R., Pharmacogenetics 14 (1), 1-18 (2004)
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