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PIM2 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PIM2 Antibody (C-term) AP7933a
    All lanes : Anti-PIM2 Antibody (D292) at 1:2000 dilution Lane 1: K562 whole cell lysate Lane 2: SW620 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PIM2 Antibody (C-term) AP7933a
    Western blot analysis of anti-PIM2 Antibody (C-term) (Cat.#AP7933a) in Hela cell line lysates (35ug/lane). PIM2 (arrow) was detected using the purified Pab.
  • WB - PIM2 Antibody (C-term) AP7933a
    Western blot analysis of PIM2 (arrow) using rabbit polyclonal PIM2 Antibody (D292) (Cat.#AP7933a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PIM2 gene.
  • IP - PIM2 Antibody (C-term) AP7933a
    PIM proteins were immunoprecipitated from MV4;11 cells and the agarose-protein A-immunoprecipitate complex was tested for its ability to phosphorylate BAD in vitro in the presence or absence of K00135. Phosphorylation of BAD (both on Ser112 and Ser136, detected by WB with phospho-specific antibodies) was abrogated on addition of the compound. Asterisks, strong bands corresponding to the heavy chain of the anti-PIM2 rabbit antibody recognized by the antirabbit immunoglobulin G secondary antibody. Beads alone (without anti-PIM antibodies) were incubated with the MV4;11 extract and used for the same in vitro phosphorylation reaction as a negative control.
  • IHC-P - PIM2 Antibody (C-term) AP7933a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9P1W9
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34190 Da
Antigen Region 277-308 aa
Additional Information
Gene ID 11040
Other Names Serine/threonine-protein kinase pim-2, Pim-2h, PIM2
Target/Specificity This PIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 277-308 amino acids from the C-terminal region of human PIM2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPIM2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIM2
Function Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti- apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Isoform 1 is less active in this respect. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Tissue Location Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues
Research Areas
Citations ( 0 )


Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases. Pim-2 is highly homologous to Pim-1 with similar oncogenic functions. Pim-2 overexpression promotes resistance to a host of apoptotic stimuli; its expression is negatively regulated by growth factor depletion. Increased levels of Pim-2 has also been observed in certain cancers.


Yan, B., et al., J. Biol. Chem. 278(46):45358-45367 (2003).
Baytel, D., et al., Biochim. Biophys. Acta 1442 (2-3), 274-285 (1998).

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$ 295.00
$ 99.00
Cat# AP7933a
(40 western blots)
Availability: In Stock
Bulk Size
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