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SAK Antibody (C-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP7946a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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SAK Antibody (C-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | O00444 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 108972 Da |
SAK Antibody (C-term) - Additional info | |
| Gene ID 10733 | |
| Other Names PLK4; SAK; STK18; Serine/threonine-protein kinase PLK4; Polo-like kinase 4; Serine/threonine-protein kinase 18; Serine/threonine-protein kinase Sak | |
| Target/Specificity This SAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 930~960 amino acids from the C-terminal region of human SAK. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:50~100 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions SAK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
SAK Antibody (C-term) - Protein Information | |
| Name PLK4 | |
| Synonyms SAK, STK18 | |
| Function Serine/threonine-protein kinase that plays a central role in centriole duplication. Able to trigger procentriole formation on the surface of the parental centriole cylinder, leading to the recruitment of centriole biogenesis proteins such as SASS6, CENPJ/CPAP, CCP110, CEP135 and gamma-tubulin. When overexpressed, it is able to induce centrosome amplification through the simultaneous generation of multiple procentrioles adjoining each parental centriole during S phase. Phosphorylates 'Ser-151' of FBXW5 during the G1/S transition, leading to inhibit FBXW5 ability to ubiquitinate SASS6. Its central role in centriole replication suggests a possible role in tumorigenesis, centrosome aberrations being frequently observed in tumors. Also involved in trophoblast differentiation by phosphorylating HAND1, leading to disrupt the interaction between HAND1 and MDFIC and activate HAND1. Phosphorylates CDC25C and CHEK2 | |
| Cellular Location Cytoplasm, cytoskeleton, centrosome, centriole. Nucleus, nucleolus (By similarity). Cleavage furrow Note=Associates with centrioles throughout the cell cycle According to PubMed:16244668, it is not present at cleavage furrows | |
SAK Antibody (C-term) - Related products
AP7946a: SAK Antibody (C-term)
SAK Antibody (C-term) - Application data
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Western blot analysis of anti-SAK Pab (Cat. #AP7946a) in placenta lysate. SAK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
SAK Antibody (C-term) - Research Areas
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BACKGROUND
SAK is involved in regulation of cell cycle. SAK is a key regulator of centriole duplication.
REFERENCES
Yamashita, Y., et al., J. Biol. Chem. 276(42):39012-39020 (2001). Hudson, J.W., et al., Gene 241(1):65-73 (2000).
