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GARS Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • WB - GARS Antibody (C-term) AP7952b
    All lanes : Anti-GARS Antibody (C-term) at 1:2000 dilution Lane 1: NCI-H460 whole cell lysate Lane 2: Ramos whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: HT-1080 whole cell lysate Lane 5: C6 whole cell lysate Lane 6: mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P41250
Other Accession NP_002038
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 83166 Da
Antigen Region 706-739 aa
Additional Information
Gene ID 2617
Other Names Glycine--tRNA ligase, Diadenosine tetraphosphate synthetase, AP-4-A synthetase, Glycyl-tRNA synthetase, GlyRS, GARS
Target/Specificity This GARS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 706-739 amino acids from the C-terminal region of human GARS.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGARS Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name GARS1 (HGNC:4162)
Synonyms GARS
Function Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) (PubMed:17544401, PubMed:28675565, PubMed:24898252). Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis (PubMed:19710017).
Cellular Location Cytoplasm. Cell projection, axon. Secreted {ECO:0000250|UniProtKB:Q9CZD3}. Secreted, extracellular exosome {ECO:0000250|UniProtKB:Q9CZD3}. Note=In transfected COS7 cells, not detected in mitochondria, nor in Golgi apparatus (PubMed:17035524) Secreted by motor neuron, possibly through the exosome pathway (By similarity). {ECO:0000250|UniProtKB:Q9CZD3, ECO:0000269|PubMed:17035524} [Isoform 2]: Cytoplasm. Cell projection, axon
Tissue Location Widely expressed, including in brain and spinal cord. [Isoform 1]: Expressed in brain, spinal cord, muscle, heart, spleen and liver.
Research Areas
Citations (0)
citation

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Background

GARS is a glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. This protein is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. The protein has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.

References

Shiba K., Schimmel P.J. Biol. Chem. 269:30049-30055(1994) Antonellis A., Ellsworth R.E.Am. J. Hum. Genet. 72:1293-1299(2003)

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$ 365.00
$ 140.00
Cat# AP7952b
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