|Application ||WB, IHC-P, E|
|Calculated MW||48112 Da|
|Antigen Region||325-356 aa|
|Other Names||Serine/threonine-protein kinase 25, Ste20-like kinase, Sterile 20/oxidant stress-response kinase 1, SOK-1, Ste20/oxidant stress response kinase 1, STK25, SOK1, YSK1|
|Target/Specificity||This YSK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 325-356 amino acids from the C-terminal region of human YSK.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||YSK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration.|
|Cellular Location||Cytoplasm. Golgi apparatus. Note=Localizes to the Golgi apparatus|
|Tissue Location||Ubiquitously expressed. Highest levels are found in testis, large intestine, brain and stomach followed by heart and lung|
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Provided below are standard protocols that you may find useful for product applications.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.
Osada, S., et al., Oncogene 14(17):2047-2057 (1997).
Pombo, C.M., et al., EMBO J. 15(17):4537-4546 (1996).
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