|Application ||WB, IHC-P, E|
|Other Accession||Q63562, Q07174|
|Calculated MW||52925 Da|
|Antigen Region||368-397 aa|
|Other Names||Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2, MAP3K8, COT, ESTF|
|Target/Specificity||This COT (MAP3K8/MEKK8) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 368-397 amino acids from the C-terminal region of human COT (MAP3K8/MEKK8).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Precautions||COT (MAP3K8/MEKK8) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Required for lipopolysaccharide (LPS)-induced, TLR4- mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the proinflammatory cytokine TNF- alpha (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. In vitro, activates MAPK/ERK pathway in response to IL1 in an IRAK1-independent manner, leading to up-regulation of IL8 and CCL4. Transduces CD40 and TNFRSF1A signals that activate ERK in B- cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle. Isoform 1 shows some transforming activity, although it is much weaker than that of the activated oncogenic variant.|
|Tissue Location||Expressed in several normal tissues and human tumor-derived cell lines|
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Provided below are standard protocols that you may find useful for product applications.
COT, a member of the MAPKKK subfamily of Ser/Thr protein kinases, is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. It plays a role in the cell cycle. The longer form of cot has some transforming activity, although it is much weaker than the activated cot oncoprotein. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
Sanchez-Gongora, E., et al., J. Biol. Chem. 275(40):31379-31386 (2000).
Aoki, M., et al., J. Biol. Chem. 268(30):22723-22732 (1993).
Chan, A.M., et al., Oncogene 8(5):1329-1333 (1993).
Miyoshi, J., et al., Mol. Cell. Biol. 11(8):4088-4096 (1991).
Aoki, M., et al., Oncogene 6(9):1515-1519 (1991).
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