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> home > Products > Antibodies > Biological Process > Protein Amino Acid Phosphorylation > MAP2K1 Antibody (T385)

MAP2K1 Antibody (T385)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Catalog #	Size	Availability	Price
AP7960g	0.1 mg 400 ul	In Stock	$ 255.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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MAP2K1 Antibody (T385) - Product info
Application	WB, IHC Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	Q02750
Reactivity	Human
Concentration	0.25 mg/ml
Isotype	Rabbit Ig
Calculated MW	43439 Da
MAP2K1 Antibody (T385) - Additional info
Gene ID 5604
Other Names MAP2K1; MEK1; PRKMK1; Dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAPK/ERK kinase 1
Target/Specificity This MAP2K1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T385 of human MAP2K1.
Dilution WB~~1:50~100WB~~1:1000 IHC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions MAP2K1 Antibody (T385) is for research use only and not for use in diagnostic or therapeutic procedures.
MAP2K1 Antibody (T385) - Protein Information
Name MAP2K1
Synonyms MEK1, PRKMK1
Function Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator- activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis
Cellular Location Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle pole body. Cytoplasm. Nucleus Note=Localizes at centrosomes during prometaphase, midzone during anaphase and midbody during telophase/cytokinesis
Tissue Location Widely expressed, with extremely low levels in brain

MAP2K1 Antibody (T385) - Related products

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AP3317a: Phospho-MAP2K1-S217 Antibody

AP3318a: Phospho-MAP2K1-T291 Antibody

AP3319a: Phospho-MAP2K1-S297 Antibody

AP3320a: Phospho-MAP2K1-T385 Antibody

AP3611a: Phospho-MEK1-pS218 Antibody

AP3612a: Phospho-MEK1-pS222 Antibody

AP7904b: MAP2K1(S218)/MAP2K2 (S216) Antibody

AP7960a: MEK1 (MAP2K1) Antibody (N-term)

AP7960c: MEK1 Antibody (Center)

AP7960d: MEK1 (MAP2K1) Antibody (S218)

AP7960e: MAP2K1 Antibody (T291)

AP7960f: MAP2K1 Antibody (S297)

AP7960g: MAP2K1 Antibody (T385)

AP7960h: MEK1 Antibody (S218/222)

AP7960i: MAP2K1 Antibody (T285)

AP7960j: MAP2K1 Antibody ( S221 )

RI13154: MAP2K1 predesign siRNA

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LY10623a: MAP2K1 Over-expression Lysate

BP3158a: Phospho-MEK1/2-S218/222 Antibody Blocking Peptide

BP3159a: Phospho-MEK1-S217/221 Antibody Blocking Peptide

BP3317a: Phospho-MAP2K1-S217 Antibody Blocking Peptide

BP3318a: Phospho-MAP2K1-T291 Antibody Blocking Peptide

BP3319a: Phospho-MAP2K1-S297 Antibody Blocking Peptide

BP3320a: Phospho-MAP2K1-T385 Antibody Blocking Peptide

BP3611a: Phospho-MEK1-pS218 Antibody Blocking Peptide

BP3612a: Phospho-MEK1-pS222 Antibody Blocking Peptide

BP7904b: MEK1 Antibody (S217) Blocking Peptide

BP7960a: MAP2K1 Antibody (N-term) Blocking Peptide

BP7960c: MEK1 Antibody (Center) Blocking Peptide

BP7960d: MAP2K1 Antibody (S217) Blocking Peptide

BP7960e: MAP2K1 Antibody (T291) Blocking Peptide

BP7960f: MAP2K1 Antibody (S297) Blocking Peptide

BP7960g: MAP2K1 Antibody (T385) Blocking Peptide

BP7960h: MEK1 Antibody (S218/222) Blocking Peptide

BP7960i: MAP2K1 Antibody (T285) Blocking Peptide

BP7960j: MAP2K1 Antibody ( S221 ) Blocking Peptide

AJ1468a: MEK1 Antibody (C-term)

AJ1468b: MEK1 Antibody (N-term)

AJ1468c: MEK1 Antibody Phospho (pT286)

AJ1468d: MEK1 Antibody Phospho (pT292)

AJ1468e: MEK1/MEK2 Antibody Phospho (pS218/222)

MAP2K1 Antibody (T385) - Application data

Western blot analysis of MAP2K1 (arrow) using rabbit polyclonal MAP2K1 Antibody (T385) (Cat.#AP7960g). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K1 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MAP2K1 Antibody (T385) (Cat.#AP7960g), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

MAP2K1 Antibody (T385) - Research Areas

Antibodies Biological Process Protein Amino Acid Phosphorylation Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Phosphorylation Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Positive Regulation Of Biological Process Positive Regulation Of Cellular Process Response To Organic Substance Positive Regulation Of Cellular Metabolic Process Positive Regulation Of Metabolic Process Positive Regulation Of Macromolecule Metabolic Process Regulation Of Phosphorylation Negative Regulation Of Cellular Process Negative Regulation Of Biological Process Response To Chemical Stimulus Regulation Of Phosphate Metabolic Process Regulation Of Phosphorus Metabolic Process Response To Endogenous Stimulus System Development Macromolecule Metabolic Process Intracellular Signaling Cascade

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BACKGROUND

MAP2K1 is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

REFERENCES

Zheng, B., et al., Blood 102(3):1019-1027 (2003). Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003). Zhu, X., et al., J. Neurochem. 86(1):136-142 (2003). Fringer, J., et al., J. Biol. Chem. 278(23):20612-20617 (2003). Witowsky, J.A., et al., J. Biol. Chem. 278(3):1403-1406 (2003).