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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MAP2K2 Antibody (S222)   

MAP2K2 Antibody (S222)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

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  • WB - MAP2K2 Antibody (S222) AP7961e
    All lanes : Anti-MAP2K2 Antibody (S222) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - MAP2K2 Antibody (S222) AP7961e
    Western blot analysis of MAP2K2-S222 (Cat. #AP7961e) in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.
  • WB - MAP2K2 Antibody (S222) AP7961e
    Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222 (Cat. #AP7961e). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2) .
  • IHC-P - MAP2K2 Antibody (S222) AP7961e
    Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) (Cat.#AP7961e), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P36507
Other Accession P36506, Q63932, Q90891, Q05116, Q01986, P29678, P31938, Q02750, Q63980, Q24324
Reactivity Human
Predicted Drosophila, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 44424 Da
Antigen Region 200-229 aa
Additional Information
Gene ID 5605
Other Names Dual specificity mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK 2, ERK activator kinase 2, MAPK/ERK kinase 2, MEK 2, MAP2K2, MEK2, MKK2, PRKMK2
Target/Specificity This MAP2K2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-229 amino acids from human MAP2K2.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMAP2K2 Antibody (S222) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP2K2
Synonyms MEK2, MKK2, PRKMK2
Function Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity).
Research Areas
Citations (0)

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MAP2K2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.


Burroughs, K.D., et al., Mol. Cancer Res. 1(4):312-322 (2003).
Tran, H., et al., Mol. Cell. Biol. 23(20):7177-7188 (2003).
Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).
Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003).
Liu, X., et al., J. Biol. Chem. 277(42):39312-39319 (2002).

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$ 295.00
$ 99.00
Cat# AP7961e
(40 western blots)
Availability: In Stock
Bulk Size
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