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SEMA3E Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - SEMA3E Antibody (C-term) AP7976b
    Western blot analysis of Semaphorin 3E(Sema3e) (C-term) (Cat. #AP7976b) in CEM cell line lysates (35ug/lane).Sema3e (arrow) was detected using the purified Pab.
  • WB - SEMA3E Antibody (C-term) AP7976b
    Western blot analysis of Semaphorin 3E(Sema3e) (C-term) (Cat. #AP7976b) in mouse lung tissue lysates (35ug/lane). Sema3e (arrow) was detected using the purified Pab.
  • IHC-P - SEMA3E Antibody (C-term) AP7976b
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Semaphorin 3E(Sema3e) (C-term) (Cat.#AP7976b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • SPECIFICATION
  • CITATIONS: 1
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession P70275
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 89543 Da
Antigen Region 737-766 aa
Additional Information
Gene ID 20349
Other Names Semaphorin-3E, Semaphorin-H, Sema H, Sema3e, Semah, Semh
Target/Specificity This SEMA3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-766 amino acids from the C-terminal region of human SEMA3E.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSEMA3E Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Sema3e
Synonyms Semah, Semh
Function Plays an important role in signaling via the cell surface receptor PLXND1. Mediates reorganization of the actin cytoskeleton, leading to the retraction of cell projections. Promotes focal adhesion disassembly and inhibits adhesion of endothelial cells to the extracellular matrix. Regulates angiogenesis, both during embryogenesis and after birth. Can down- regulate sprouting angiogenesis. Required for normal vascular patterning during embryogenesis. Plays an important role in ensuring the specificity of synapse formation.
Cellular Location Secreted.
Tissue Location Detected in neurons in the thalamus. Detected in embryonic vasculature. Developing lungs, developing skeletal elements and ventral horns of the developing neural tube Correlates positively with tumor progression
Research Areas
Citations ( 0 )

Background

The Semaphorins make up the largest family of axon guidance cues yet described. Semaphorins are divided into 8 classes (classes 3-7 found in vertebrates). Class 3 Semaphorins are secreted, classes 4 through 6 are transmembrane proteins, and class 7 are membrane associated via glycosylphosphatidylinositol (GPI) linkage. They are characterized structurally by a conserved ~400 amino acid sema domain. They are classically described as collapsing factors and mediators of axon repulsion, although they may also act as context-dependent chemoattractants. Semaphorins have been shown to have roles in cardiovascular development and in the regulation of immune cell antigen presentation. Receptors or receptor complexes that mediate semaphorin signaling include proteins of the Neuropilin and Plexin families.

References

Christensen C.R.L.,Cancer Res. 58:1238-1244(1998).

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Discontinued
Cat# AP7976b
Size:
(40 western blots)

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