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Tiparp Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • IHC-P - Tiparp  Antibody (N-term) AP7978a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Tiparp antibody (N-term) (Cat.#AP7978a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • WB - Tiparp  Antibody (N-term) AP7978a
    Tiparp Antibody (N-term) (Cat.#AP7978a) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the Tiparp antibody detected the Tiparp protein (arrow).
  • SPECIFICATION
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession Q8C1B2
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 75901 Da
Antigen Region 60-90 aa
Additional Information
Gene ID 99929
Other Names TCDD-inducible poly [ADP-ribose] polymerase, ADP-ribosyltransferase diphtheria toxin-like 14, ARTD14, Tiparp
Target/Specificity This Tiparp antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-90 amino acids from the N-terminal region of human Tiparp.
Dilution IHC-P~~1:10~50
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsTiparp Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Tiparp
Function Poly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptive response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.
Tissue Location Ubiquitously expressed.
Citations (0)

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Background

Poly [ADP-ribose] polymerase using NAD(+) as a substrate to transfer ADP-ribose onto glutamic acid residues of a protein acceptor; repeated rounds of ADP-ribosylation leads to the formation of poly(ADPribose) chains on the protein, thereby altering the function of the target protein. May play a role in the adaptative response to chemical exposure (TCDD) and thereby mediates certain effects of the chemicals.

References

Schmahl,J., Nat. Genet. 39 (1), 52-60 (2007)
Ma,Q., Arch. Biochem. Biophys. 404 (2), 309-316 (2002)
Ma,Q., Biochem. Biophys. Res. Commun. 289 (2), 499-506 (2001)

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Cat# AP7978a
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(40 western blots)
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