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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NFKBIA Antibody ( S32/36 )   

NFKBIA Antibody ( S32/36 )

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NFKBIA Antibody ( S32/36 ) AP7981b
    Western blot analysis of NFKBIA-S32/36 (Cat. #AP7981b) in Hela cell line lysates (35ug/lane). NFKBIA (arrow) was detected using the purified Pab.
  • IHC-P - NFKBIA Antibody ( S32/36 ) AP7981b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma with NFKBIA Antibody (S32/36 ), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • FC - NFKBIA Antibody ( S32/36 ) AP7981b
    Flow cytometric analysis of hela cells using NFKBIA Antibody ( S32/36 )(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
  • IF - NFKBIA Antibody ( S32/36 ) AP7981b
    Confocal immunofluorescent analysis of NFKBIA Antibody ( S32/36 )(Cat#AP7981b) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P25963
Other Accession Q08353, Q9Z1E3
Reactivity Human
Predicted Mouse, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 35609 Da
Antigen Region 12-41 aa
Additional Information
Gene ID 4792
Other Names NF-kappa-B inhibitor alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, Major histocompatibility complex enhancer-binding protein MAD3, NFKBIA, IKBA, MAD3, NFKBI
Target/Specificity This NFKBIA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-41 amino acids from human NFKBIA.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNFKBIA Antibody ( S32/36 ) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms IKBA, MAD3, NFKBI
Function Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Cellular Location Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
Research Areas
Citations (0)

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NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).


Szamosi,T., Dig. Dis. Sci. (2008)
Fan,C., J. Biol. Chem. 278 (3), 2072-2080 (2003)
Tojima,Y., Nature 404 (6779), 778-782 (2000)
Gil,J., Oncogene 19 (11), 1369-1378 (2000)
Hay,R.T., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 354 (1389), 1601-1609(1999)

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Cat# AP7981b
(40 western blots)
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