- CITATIONS: 8
|Application ||WB, IHC-P, E|
|Calculated MW||87432 Da|
|Antigen Region||714-744 aa|
|Other Names||cGMP-dependent protein kinase 2, cGK 2, cGK2, cGMP-dependent protein kinase II, cGKII, PRKG2, PRKGR2|
|Target/Specificity||This cGKII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 714-744 amino acids from the C-terminal region of human cGKII.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||cGKII Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Cellular Location||Apical cell membrane; Lipid-anchor|
|Tissue Location||Highly concentrated in brain, lung and intestinal mucosa|
Provided below are standard protocols that you may find useful for product applications.
cGKII is thought to play a key role in a diverse set of physiological pathway. cGKII may mediate intestinal secretion of water and electrolytes induced by the E. coli toxin STa and the intestinal peptide guanylin. Edentification of the pathway that mediates intestinal fluid secretion by E. coli STa has potential medical implications because STa causes traveler's diarrhea and about 50% of infant mortality in developing countries. Transfection experiments in human cells disclose that cGKII phosphorylates SOX9 and attenuates SOX9 function by inhibiting its nuclear entry. Impaired differentiation of cultured KMI chondrocytes can be restored by silencing Sox9 by RNA interference. cGKII is postulated to be a molecular switch that couples the cessation of proliferation and the start of hypertrophic chondrocyte differentiation through attenuating SOX9 function.
Vaandrager, A.B., et al., J. Biol. Chem. 278(31):28651-28658 (2003).
Gambaryan, S., et al., Biochem. Biophys. Res. Commun. 293(5):1438-1444 (2002).
Orstavik, S., et al., Biochem. Biophys. Res. Commun. 220(3):759-765 (1996).
Fujii, M., et al., FEBS Lett. 375(3):263-267 (1995).
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