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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NIK Antibody (C-term)   

NIK Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - NIK Antibody (C-term) AP8004a
    Western blot analysis of anti-HGK Antibody (C-term)(Cat.#AP8004a) in jurkat cell line lysates (35ug/lane). HGK(arrow) was detected using the purified Pab.
  • SPECIFICATION
  • CITATIONS: 4
  • PROTOCOLS
  • BACKGROUND
Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession O95819
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 142101 Da
Antigen Region 618-649 aa
Additional Information
Gene ID 9448
Other Names Mitogen-activated protein kinase kinase kinase kinase 4, HPK/GCK-like kinase HGK, MAPK/ERK kinase kinase kinase 4, MEK kinase kinase 4, MEKKK 4, Nck-interacting kinase, MAP4K4, HGK, KIAA0687, NIK
Target/Specificity This NIK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 618-649 amino acids from the C-terminal region of human NIK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNIK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MAP4K4
Synonyms HGK, KIAA0687, NIK
Function Serine/threonine kinase that may play a role in the response to environmental stress and cytokines such as TNF-alpha. Appears to act upstream of the JUN N-terminal pathway. Phosphorylates SMAD1 on Thr-322.
Cellular Location Cytoplasm.
Tissue Location Appears to be ubiquitous. Expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain. Isoform 4 is predominant in the liver, skeletal muscle and placenta.
Research Areas
Citations ( 0 )

Background

HGK, a member of the STE20 subfamily of Ser/Thr protein kinases, may play a role in the response to environmental stress and cytokines such as TNF-alpha. It appears to act upstream of the JUN N-terminal pathway. This protein is thought to interact with the SH3 domain of the adapter proteins Nck. HGK binds, via its CNH regulatory domain, to the N-terminal region of SPG3A. Expression appears to be ubiquitous, expressed in all tissue types examined. Isoform 5 appears to be more abundant in the brain, and isoform 4 is predominant in the liver, skeletal muscle and placenta.

References

Wright, J.H., et al., Mol. Cell. Biol. 23(6):2068-2082 (2003). Yao, Z., et al., J. Biol. Chem. 274(4):2118-2125 (1999). Ishikawa, K., et al., DNA Res. 5(3):169-176 (1998).

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$ 295.00
$ 99.00
Cat# AP8004a
Size:
Quantity:
(40 western blots)
Availability: In Stock
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