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PI3KC2G Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - PI3KC2G Antibody (N-term) AP8012a
    Western blot analysis of PI3KC2G antibody (N-term) (Cat.# AP8012a) in CEM cell line lysates (35ug/lane). PI3KC2G (arrow) was detected using the purified Pab.
    detail
  • IHC-P - PI3KC2G Antibody (N-term) AP8012a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
IHC-P, WB, E
Primary Accession O75747
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 170682 Da
Antigen Region 93-123 aa
Additional Information
Gene ID 5288
Other Names Phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit gamma, PI3K-C2-gamma, PtdIns-3-kinase C2 subunit gamma, Phosphoinositide 3-kinase-C2-gamma, PIK3C2G
Target/Specificity This PI3KC2G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-123 amino acids from the N-terminal region of human PI3KC2G.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI3KC2G Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3C2G
Function Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers (By similarity). May play a role in SDF1A-stimulated chemotaxis (By similarity).
Cellular Location Membrane {ECO:0000250|UniProtKB:O70167}; Peripheral membrane protein {ECO:0000250|UniProtKB:O70167}
Tissue Location Highly expressed in liver, prostate and testis. Lower levels in small intestine, kidney and pancreas
Research Areas
Citations (0)
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Background

PI3KC2G belongs to the phosphoinositide 3-kinase (PI3K) family. PI3-kinases play roles in signaling pathways involved in cell proliferation, oncogenic transformation, cell survival, cell migration, and intracellular protein trafficking. This protein contains a lipid kinase catalytic domain as well as a C-terminal C2 domain, a characteristic of class II PI3-kinases. C2 domains act as calcium-dependent phospholipid binding motifs that mediate translocation of proteins to membranes, and may also mediate protein-protein interactions. The biological function of this gene has not yet been determined.

References

Rozycka, M., et al., Genomics 54(3):569-574 (1998).

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$ 365.00
$ 140.00
Cat# AP8012a
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