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PI3KC3 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
United StatesOrdering Information
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(40 western blots)
$ 265.00email & fax$ 251.75online
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(8 western blots)
$ 95.00email & fax$ 90.25online
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- Citations : 3
PI3KC3 Antibody (N-term) - Product info
|Application ||WB, IHC, IF|
|Other Accession||Q6AZN6, O88763, Q5D891, Q6PF93|
|Predicted||Mouse, Rat, Pig, Xenopus|
|Calculated MW||101549 Da|
PI3KC3 Antibody (N-term) - Additional info
|Gene ID 5289|
PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PI3KC3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
PI3KC3 Antibody (N-term) - Protein Information
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity)
Ubiquitously expressed, with a highest expression in skeletal muscle
PI3KC3 Antibody (N-term) - Related products
PI3KC3 Antibody (N-term) - Research Areas
Western blot analysis of anti-PI3KC3 Pab (Cat. #AP8014a) in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term)(Cat#AP8014a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Induction of autophagy is essential for monocyte-macrophage differentiation.
Author : Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG.
Blood. 2012 Mar 22;119(12):2895-905. doi: 10.1182/blood-2011-08-372383. Epub 2012 Jan 5.
PubMed® Id : 22223827
Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors?
Author : Miselli F, Negri T, Gronchi A, Losa M, Conca E, Brich S, Fumagalli E, Fiore M, Casali PG, Pierotti MA, Tamborini E, Pilotti S.
Transl Oncol. 2008 Dec;1(4):177-86.
PubMed® Id : 19043528
Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG.
Author : Liang C, Feng P, Ku B, Dotan I, Canaani D, Oh BH, Jung JU.
Nat Cell Biol. 2006 Jul;8(7):688-99. Epub 2006 Jun 25.
PubMed® Id : 16799551
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Provided below are standard protocols that you may find useful for product applications.
PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).