PI3KC3 Antibody (N-term)
Purified Rabbit Polyclonal Antibody (Pab)
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(40 western blots)
|In Stock||$ 265.00||Add to cart|
(8 western blots)
|In Stock||$ 95.00||Add to cart|
- Citations : 6
PI3KC3 Antibody (N-term) - Product Information
|Application ||WB, IHC, IF|
|Other Accession||Q6AZN6, O88763, Q5D891, Q6PF93|
|Predicted||Mouse, Pig, Rat, Xenopus|
|Calculated MW||101549 Da Da|
|Antigen Region||24-53 aa|
PI3KC3 Antibody (N-term) - Additional Information
|Gene ID 5289|
Phosphatidylinositol 3-kinase catalytic subunit type 3, PI3-kinase type 3, PI3K type 3, PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, Phosphoinositide-3-kinase class 3, hVps34, PIK3C3, VPS34
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PI3KC3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
PI3KC3 Antibody (N-term) - Protein Information
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme.
Ubiquitously expressed, with a highest expression in skeletal muscle.
PI3KC3 Antibody (N-term) - Related Products
PI3KC3 Antibody (N-term) - Research Areas
Western blot analysis of lysates from Hela, SH-SY5Y cell line (from left to right), using PI3KC3 Antibody (N-term)(Cat. #AP8014A). AP8014A was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
Induction of autophagy is essential for monocyte-macrophage differentiation.
Author : Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG.
Blood. 2012 Mar 22;119(12):2895-905. doi: 10.1182/blood-2011-08-372383. Epub 2012 Jan 5.
PubMed® Id : 22223827
The class III kinase Vps34 promotes T lymphocyte survival through regulating IL-7Rα surface expression.
Author : McLeod IX1, Zhou X, Li QJ, Wang F, He YW.
J Immunol. 2011 Nov 15;187(10):5051-61. doi: 10.4049/jimmunol.1100710. Epub 2011 Oct 21.
PubMed® Id : 22021616
Deletion of PIK3C3/Vps34 in sensory neurons causes rapid neurodegeneration by disrupting the endosomal but not the autophagic pathway.
Author : Zhou X1, Wang L, Hasegawa H, Amin P, Han BX, Kaneko S, He Y, Wang F.
Proc Natl Acad Sci U S A. 2010 May 18;107(20):9424-9. doi: 10.1073/pnas.0914725107. Epub 2010 May 3.
PubMed® Id : 20439739
A non-canonical MEK/ERK signaling pathway regulates autophagy via regulating Beclin 1.
Author : Wang J1, Whiteman MW, Lian H, Wang G, Singh A, Huang D, Denmark T.
J Biol Chem. 2009 Aug 7;284(32):21412-24. doi: 10.1074/jbc.M109.026013. Epub 2009 Jun 11.
PubMed® Id : 19520853
Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors?
Author : Miselli F, Negri T, Gronchi A, Losa M, Conca E, Brich S, Fumagalli E, Fiore M, Casali PG, Pierotti MA, Tamborini E, Pilotti S.
Transl Oncol. 2008 Dec;1(4):177-86.
PubMed® Id : 19043528
Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG.
Author : Liang C, Feng P, Ku B, Dotan I, Canaani D, Oh BH, Jung JU.
Nat Cell Biol. 2006 Jul;8(7):688-99. Epub 2006 Jun 25.
PubMed® Id : 16799551
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Provided below are standard protocols that you may find useful for product applications.
PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.
Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).