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PI3KC3 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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Ordering Information
Catalog # SizeAvailability Price  
AP8014a 400 µl
(40 western blots)
In Stock
$ 265.00email & fax$ 251.75online
Add to cart
AP8014a-ev 80 µl
(8 western blots)
In Stock
$ 95.00email & fax$ 90.25online
Add to cart
  • Specification
  • Citations : 3
  • Reviews
  • Protocols
  • Background

PI3KC3 Antibody (N-term) - Product info

Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IF
Primary AccessionQ8NEB9
Other AccessionQ6AZN6, O88763, Q5D891, Q6PF93
ReactivityHuman
PredictedMouse, Rat, Pig, Xenopus
Isotype Rabbit Ig
Calculated MW 101549 Da

PI3KC3 Antibody (N-term) - Additional info

Gene ID 5289
Other Names
PIK3C3; VPS34; Phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase p100 subunit; Phosphoinositide-3-kinase class 3; hVps34
Target/Specificity
This PI3KC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 24-53 amino acids from the N-terminal region of human PI3KC3.
Dilution
WB~~1:1000
IHC~~1:50~100
IF~~1:10~50
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
PI3KC3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

PI3KC3 Antibody (N-term) - Protein Information

Name PIK3C3
Synonyms VPS34
Function
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which plays a key role in initiation and maturation of autophagosomes. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for the abcission step in cytokinesis. Required for transport from early to late endosomes.
Cellular Location
Midbody. Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (By similarity)
Tissue Location
Ubiquitously expressed, with a highest expression in skeletal muscle

PI3KC3 Antibody (N-term) - Related products

AP1851a: PI3KC3 Antibody (N-term G24)

AP1851b: PI3KC3 Antibody (C-term E785)

AP1851E: PI3KC3 Antibody (S34)

AP1851g: PI3KC3 Antibody (S164)

AP1851h: PI3KC3 Antibody (S425)

AP1851j: PI3KC3 Antibody (S851)

AP3368a: Phospho-PI3KC3(S282) Antibody

AP3369a: Phospho-PI3KC3(S676) Antibody

AP3399a: Phospho-PI3KC3(S164) Antibody

BP1851b: Kinase PI3KC3 Antibody (C-term) Blocking Peptide

BP1851e: PI3KC3 Antibody (S34) Blocking Peptide

BP1851g: PI3KC3 Antibody (S164) Blocking Peptide

BP1851h: PI3KC3 Antibody (S425) Blocking Peptide

BP1851j: PI3KC3 Antibody (S851) Blocking Peptide

BP3368a: Phospho-PI3KC3-S282 Antibody Blocking Peptide

BP3369a: Phospho-PI3KC3-S676 Antibody Blocking Peptide

BP3399a: Phospho-PI3KC3-S164 Antibody Blocking Peptide

BP8014a: PI3KC3 Antibody (N-term) Blocking Peptide

PI3KC3 Antibody (N-term) - Research Areas

Application data

  • WB - PI3KC3 Antibody (N-term) AP8014a

    Western blot analysis of anti-PI3KC3 Pab (Cat. #AP8014a) in HeLa cell lysate. PI3KC3 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

  • IHC - PI3KC3 Antibody (N-term) AP8014a

    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

  • IF - PI3KC3 Antibody (N-term) AP8014a

    Confocal immunofluorescent analysis of PI3KC3 Antibody (N-term)(Cat#AP8014a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

  • WB - PI3KC3 Antibody (N-term) AP8014a
  • IHC - PI3KC3 Antibody (N-term) AP8014a
  • IF - PI3KC3 Antibody (N-term) AP8014a

ELITE™ Custom Antibody Services

  • Induction of autophagy is essential for monocyte-macrophage differentiation. Author : Zhang Y, Morgan MJ, Chen K, Choksi S, Liu ZG.
    Blood. 2012 Mar 22;119(12):2895-905. doi: 10.1182/blood-2011-08-372383. Epub 2012 Jan 5.
    PubMed® Id : 22223827
  • Is autophagy rather than apoptosis the regression driver in imatinib-treated gastrointestinal stromal tumors? Author : Miselli F, Negri T, Gronchi A, Losa M, Conca E, Brich S, Fumagalli E, Fiore M, Casali PG, Pierotti MA, Tamborini E, Pilotti S.
    Transl Oncol. 2008 Dec;1(4):177-86.
    PubMed® Id : 19043528
  • Autophagic and tumour suppressor activity of a novel Beclin1-binding protein UVRAG. Author : Liang C, Feng P, Ku B, Dotan I, Canaani D, Oh BH, Jung JU.
    Nat Cell Biol. 2006 Jul;8(7):688-99. Epub 2006 Jun 25.
    PubMed® Id : 16799551

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Provided below are standard protocols that you may find useful for product applications.

BACKGROUND

PI3KC3 is a catalytic subunit of the PI3K complex involved in the transport of lysosomal enzyme precursors to lysosomes. This enzyme acts catalytically to convert 1-phosphatidyl-1D-myo-inositol to 1-phosphatidyl-1D-myo-inositol 3-phosphate. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). The regulation of the Beclin 1-PI3KC3 complex lipid kinase activity is a critical element in the autophagy signaling pathway.

REFERENCES

Vergne, I., et al., J. Exp. Med. 198(4):653-659 (2003). Volinia, S., et al., EMBO J. 14(14):3339-3348 (1995).