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> home > Products > Antibodies > Biological Process > Protein Amino Acid Phosphorylation > PI3KCA Antibody (Center)

PI3KCA Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)

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Catalog #	Size	Availability	Price
AP8016c	0.1 mg 400 ul	In Stock	$ 255.00	DISCONTINED INQUIRE CLICK INQUIRE Add to cart

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PI3KCA Antibody (Center) - Product info
Application	WB, IHC Applications Legend: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Primary Accession	P42336
Other Accession	Q14CW1
Reactivity	Human
Concentration	0.25 mg/ml
Isotype	Rabbit Ig
Calculated MW	124284 Da
PI3KCA Antibody (Center) - Additional info
Gene ID 5290
Other Names PIK3CA; Phosphatidylinositol 4, 5-bisphosphate 3-kinase catalytic subunit alpha isoform; Phosphatidylinositol 4, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha; Phosphoinositide-3-kinase catalytic alpha polypeptide; Serine/threonine protein kinase PIK3CA
Target/Specificity This PI3KCA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 511~541 amino acids from the the center region of human PI3KCA.
Dilution WB~~1:50~100WB~~1:1000 IHC~~1:50-100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions PI3KCA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
PI3KCA Antibody (Center) - Protein Information
Name PIK3CA
Function Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4- phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5- bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation in breast cancer cells through the PDPK1- AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway Has also serine-protein kinase activity: phosphorylates PIK3R1 (p85alpha regulatory subunit), EIF4EBP1 and HRAS

PI3KCA Antibody (Center) - Related products

AP8016c: PI3KCA Antibody (Center)

AP3558a: Phospho-PK3CA-pY317 Antibody

AP7860a: PK3CA Antibody (Y317)

AP8016a: PI3KCA Antibody (N-term)

AP8016b: PI3KCA Antibody (C-term)

RI13994: PIK3CA predesign siRNA

BP3558a: Phospho-PK3CA-pY317 Antibody Blocking Peptide

BP7860a: PK3CA Antibody (Y317) Blocking Peptide

BP8016a: PI3KCA Antibody (N-term) Blocking Peptide

BP8016b: PI3KCA Antibody (C-term) Blocking Peptide

BP8016c: PI3KCA Antibody (Center) Blocking Peptide

AJ1607a: PI3-kinase (p110) Antibody (subunit alpha)

AF1827a: PIK3CA / P110alpha Antibody

PI3KCA Antibody (Center) - Application data

Western blot analysis of anti-PIK3CA Antibody (Center) Pab (Cat.#AP8016c) in K562 cell line lysates (35ug/lane). PIK3CA (arrow) was detected using the purified Pab (1:60 dilution)
Western blot analysis of anti-PI3KCA Antibody (Center) Pab (Cat.#AP8016c) in 293 cell line lysates transiently transfected with the PIK3CA gene (1ug/lane). PI3KCA (arrow) was detected using the purified Pab (1:60 dilution).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCA Antibody (Center)(Cat.#AP8016c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

PI3KCA Antibody (Center) - Research Areas

Antibodies Biological Process Protein Amino Acid Phosphorylation Post-translational Protein Modification Phosphate Metabolic Process Phosphorus Metabolic Process Phosphorylation Protein Modification Process Biopolymer Modification Metabolic Process Primary Metabolic Process Protein Metabolic Process Cellular Protein Metabolic Process Cellular Metabolic Process Cellular Process Regulation Of Cell Death Regulation Of Programmed Cell Death Regulation Of Apoptosis Lipid Metabolic Process Negative Regulation Of Cellular Process Negative Regulation Of Biological Process Macromolecule Metabolic Process Intracellular Signaling Cascade Protein Kinase Cascade Negative Regulation Of Apoptosis Negative Regulation Of Cell Death Negative Regulation Of Programmed Cell Death Cellular Macromolecule Metabolic Process Alcohol Metabolic Process Cellular Lipid Metabolic Process Regulation Of Multicellular Organismal Process Hexose Metabolic Process Anti-apoptosis Monosaccharide Metabolic Process

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BACKGROUND

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns(4,5)P2. This gene has been found to be oncogenic and has been implicated in cervical cancers.

REFERENCES

Tiwari, S. Nat Immunol. August; 10(8): 907?17 (2009). Ballou, L.M., et al., J. Biol. Chem. 278(26):23472-23479 (2003). Singh, B., et al., Genes Dev. 16(8):984-993 (2002). Shayesteh, L., et al., Nat. Genet. 21(1):99-102 (1999). Volinia, S., et al., Genomics 24(3):472-477 (1994). Hiles, I.D., et al., Cell 70(3):419-429 (1992).