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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PI3KR4 Antibody (N-term)   

PI3KR4 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PI3KR4 Antibody (N-term) AP8026a
    Western blot analysis of anti-PI3KR4 Pab (Cat. #AP8026a) in T-47D cell lysate. PI3KR4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - PI3KR4 Antibody (N-term) AP8026a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q99570
Other Accession NP_055417
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 153103 Da
Antigen Region 53-83 aa
Additional Information
Gene ID 30849
Other Names Phosphoinositide 3-kinase regulatory subunit 4, PI3-kinase regulatory subunit 4, PI3-kinase p150 subunit, Phosphoinositide 3-kinase adaptor protein, PIK3R4
Target/Specificity This PI3KR4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 53-83 amino acids from the N-terminal region of human PI3KR4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI3KR4 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PIK3R4
Synonyms VPS15 {ECO:0000303|PubMed:23878393}
Function Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Cellular Location Late endosome. Note=Localizes also to discrete punctae along the ciliary axoneme
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.


Panaretou, C., et al., J. Biol. Chem. 272(4):2477-2485 (1997).

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$ 295.00
$ 99.00
Cat# AP8026a
(40 western blots)
Availability: In Stock
Bulk Size
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