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PI4K2A (PI4K II) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PI4K2A  (PI4K II) Antibody (N-term) AP8032a
    Anti-PI4K II Antibody (C-term) at 1:8000 dilution + human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - PI4K2A  (PI4K II) Antibody (N-term) AP8032a
    Western blot analysis of anti-PI4K II Pab (Cat. #AP8032a) in HL60 cell lysate. PI4K II (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • IHC-P - PI4K2A  (PI4K II) Antibody (N-term) AP8032a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • IF - PI4K2A  (PI4K II) Antibody (N-term) AP8032a
    Negative control of hela cells without Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).Alexa-Fluor-546 emits orange fluorescence. Immunofluorescence analysis of PI4K2A (PI4K II) Antibody (N-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L).
  • WB - PI4K2A  (PI4K II) Antibody (N-term) AP8032a
    Western blot analysis of anti-PI4K2A Antibody (N-term D2) (Cat.#AP7575d) in mouse brain tissue lysates (35ug/lane). PI4KII(arrow) was detected using the purified Pab.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9BTU6
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 54022 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 55361
Other Names Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha, PI4K2A
Target/Specificity This PI4K2A (PI4K II) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PI4K2A (PI4K II).
Dilution WB~~1:1000
Format Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPI4K2A (PI4K II) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PI4K2A
Function Membrane-bound phosphatidylinositol-4 kinase (PI4- kinase) that catalyzes the phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P), a lipid that plays important roles in endocytosis, Golgi function, protein sorting and membrane trafficking and is required for prolonged survival of neurons. Besides, phosphorylation of phosphatidylinositol (PI) to phosphatidylinositol 4-phosphate (PI4P) is the first committed step in the generation of phosphatidylinositol 4,5-bisphosphate (PIP2), a precursor of the second messenger inositol 1,4,5-trisphosphate (InsP3).
Cellular Location Golgi apparatus, trans-Golgi network membrane; Lipid-anchor. Membrane raft. Cell projection, dendrite {ECO:0000250|UniProtKB:Q2TBE6}. Cell junction, synapse, presynaptic cell membrane {ECO:0000250|UniProtKB:Q2TBE6}. Cell junction, synapse, synaptosome {ECO:0000250|UniProtKB:Q2TBE6} Mitochondrion {ECO:0000250|UniProtKB:Q2TBE6}. Endosome Cytoplasmic vesicle. Membrane; Lipid-anchor. Cell membrane Note=Localized in neuronal cell body. Transported from neuronal cell body to neuron projections in a BLOC-1- and AP-3-complexes- dependent manner. Enriched in neurite tips and neuron projections in a BLOC-1- and AP-3-complexes-dependent manner (By similarity) Found in subdomains of the plasma membrane termed non-caveolar membrane rafts. {ECO:0000250|UniProtKB:Q2TBE6, ECO:0000250|UniProtKB:Q99M64}
Tissue Location Widely expressed. Highest expression is observed in kidney, brain, heart, skeletal muscle, and placenta and lowest expression is observed in colon, thymus, and small intestine.
Research Areas
Citations (0)

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Phosphatidylinositolpolyphosphates (PtdInsPs) are centrally involved in many biologic processes, ranging from cell growth and organization of the actin cytoskeleton to endo- and exocytosis. PI4KII phosphorylates PtdIns at the D-4 position, an essential step in the biosynthesis of PtdInsPs. PI4K II is activated by detergent and inhibited by adenosine. Overexpression of PI4KII in COS-7 cells increases synthesis of PtdIns4P. Some cells overexpressing PI4KII have scattered or no perinuclear Golgi. Knockdown of PI4KII by RNA interference (RNAi) does not disrupt the Golgi, and some cells show expanded Golgi. RNAi reduces the Golgi level of PtdIns4P and blocks the association between AP1 and the trans-Golgi network. PI4KII RNAi had little effect on intra-Golgi trafficking, but it inhibited export to plasma membrane export by 35%. It has been proposed that PI4KII generates PtdIns4P-rich domains within the Golgi that specify docking of the AP1 coat machinery.


Wang, Y.J., et al., Cell 114(3):299-310 (2003). Minogue, S., et al., J. Biol. Chem. 276(20):16635-16640 (2001). Barylko, B., et al., J. Biol. Chem. 276(11):7705-7708 (2001). Waugh, M.G., et al., Biochem. J. 373 (Pt 1), 57-63 (2003).

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$ 295.00
$ 99.00
Cat# AP8032a
(40 western blots)
Availability: In Stock
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