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Endophilin B1 Antibody (Y80)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

  • WB - Endophilin B1 Antibody (Y80) AP8034a
    Anti-Endophilin Antibody (Y80) at 1:1000 dilution + U118MG whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - Endophilin B1 Antibody (Y80) AP8034a
    Western blot analysis of anti-Endophilin B1 Antibody (Y80) (Cat.#AP8034a) in HepG2 cell line lysates (35ug/lane). SH3GLB1 (arrow) was detected using the purified Pab.
  • IHC-P - Endophilin B1 Antibody (Y80) AP8034a
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with Endophilin B1 Antibody (Y80) (Cat.#AP8034a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9Y371
Other Accession Q6AYE2, Q9JK48, Q32LM0
Reactivity Human
Predicted Bovine, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 40796 Da
Antigen Region 59-86 aa
Additional Information
Gene ID 51100
Other Names Endophilin-B1, Bax-interacting factor 1, Bif-1, SH3 domain-containing GRB2-like protein B1, SH3GLB1, KIAA0491
Target/Specificity This Endophilin B1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-86 amino acids from human Endophilin B1.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEndophilin B1 Antibody (Y80) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SH3GLB1
Synonyms KIAA0491
Function May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion.
Cellular Location Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Mitochondrion outer membrane; Peripheral membrane protein. Note=Association with the Golgi apparatus depends on the cell type.
Tissue Location Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
Research Areas
Citations (0)

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Endophilin B1 may be required for normal outer mitochondrial membrane dynamics. It is required for coatomer-mediated retrograde transport in certain cells. It may recruit other proteins to membranes with high curvature and may promote membrane fusion.


Yamaguchi,H., J. Biol. Chem. 283 (27), 19112-19118 (2008)
Lee,J.W., Pathology 38 (4), 312-315 (2006)
Masuda,M., EMBO J. 25 (12), 2889-2897 (2006)

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$ 295.00
$ 99.00
Cat# AP8034a
(40 western blots)
Availability: In Stock
Bulk Size
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