|Application ||WB, IHC-P, E|
|Other Accession||Q9R0I8, O70172|
|Calculated MW||46225 Da|
|Antigen Region||107-139 aa|
|Other Names||Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha, 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha, Diphosphoinositide kinase 2-alpha, PIP5KIII, Phosphatidylinositol 5-phosphate 4-kinase type II alpha, PI(5)P 4-kinase type II alpha, PIP4KII-alpha, PtdIns(4)P-5-kinase B isoform, PtdIns(4)P-5-kinase C isoform, PtdIns(5)P-4-kinase isoform 2-alpha, PIP4K2A, PIP5K2, PIP5K2A|
|Target/Specificity||This PIP4KII-alpha(PIP4K2A) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-139 amino acids from the N-terminal region of human PIP4KII-alpha(PIP4K2A).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PIP4KII-alpha (PIP4K2A) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.|
|Cellular Location||Cell membrane. Nucleus. Cytoplasm. Note=May translocate from the cytosol to the cell membrane upon activation of tyrosine phosphorylation. May translocate from the inner to the outer segments of the rod photoreceptor cells in response to light (By similarity). Localization to the nucleus is modulated by the interaction with PIP4K2B.|
|Tissue Location||Expressed ubiquitously, with high levels in the brain. Present in most tissues, except notably skeletal muscle and small intestine.|
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Provided below are standard protocols that you may find useful for product applications.
Phosphatidylinositol-4,5-bisphosphate, the precursor to second messengers of the phosphoinositide signal transduction pathways, is thought to be involved in the regulation of secretion, cell proliferation, differentiation, and motility. TPIP5K2A is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-4-phosphate on the fifth hydroxyl of the myo-inositol ring to form phosphatidylinositol-4,5-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases, but the recombinant protein does exhibit kinase activity. The protein is a member of the phosphatidylinositol-4-phosphate 5-kinase family.
Rozenvayn, N., et al., J. Biol. Chem. 278(10):8126-8134 (2003).
Boronenkov, I.V., et al., J. Biol. Chem. 270(7):2881-2884 (1995).
Loijens, J.C., et al., Adv. Enzyme Regul. 36, 115-140 (1996).
Divecha, N., et al., Biochem. J. 309 (Pt 3), 715-719 (1995).
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