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DGKZ Antibody (C-term)
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application 
| IF, IHC-P, WB, E |
|---|---|
| Primary Accession | Q13574 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 103981 Da |
| Antigen Region | 989-1019 aa |
| Gene ID | 8525 |
|---|---|
| Other Names | Diacylglycerol kinase zeta, DAG kinase zeta, Diglyceride kinase zeta, DGK-zeta, DGKZ, DAGK6 |
| Target/Specificity | This DGKZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 989-1019 amino acids from the C-terminal region of human DGKZ. |
| Dilution | IF~~1:10~50 WB~~1:1000 IHC-P~~1:50~100 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | DGKZ Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | DGKZ (HGNC:2857) |
|---|---|
| Synonyms | DAGK6 |
| Function | Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095). Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (PubMed:9159104, PubMed:15544348, PubMed:18004883, PubMed:19744926, PubMed:22108654, PubMed:22627129, PubMed:23949095). Also plays an important role in the biosynthesis of complex lipids (Probable). Does not exhibit an acyl chain-dependent substrate specificity among diacylglycerol species (PubMed:9159104, PubMed:19744926, PubMed:22108654). Can also phosphorylate 1-alkyl-2-acylglycerol in vitro but less efficiently and with a preference for alkylacylglycerols containing an arachidonoyl group (PubMed:15544348, PubMed:19744926, PubMed:22627129). The biological processes it is involved in include T cell activation since it negatively regulates T-cell receptor signaling which is in part mediated by diacylglycerol (By similarity). By generating phosphatidic acid, stimulates PIP5KIA activity which regulates actin polymerization (PubMed:15157668). Through the same mechanism could also positively regulate insulin-induced translocation of SLC2A4 to the cell membrane (By similarity). |
| Cellular Location | Nucleus. Cytoplasm, cytosol. Cell membrane. Cell projection, lamellipodium |
| Tissue Location | Highest levels in brain, and substantial levels in skeletal muscle, heart, and pancreas. |
Research Areas
Citations ( 0 )
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
DGKZ belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction.
References
Hogan, A., et al., J. Biol. Chem. 276(28):26526-26533 (2001).
Topham, M.K., et al., Nature 394(6694):697-700 (1998).
Ding, L., et al., Proc. Natl. Acad. Sci. U.S.A. 94(11):5519-5524 (1997).
Bunting, M., et al., J. Biol. Chem. 271(17):10230-10236 (1996).
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$ 365.00
$ 140.00
Cat# AP8060B
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