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GNB2L1 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

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United States
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Ordering Information
Catalog # SizeAvailability Available Date  
AP8063a 400 µl
(40 western blots)
Backorder 4 months
AP8063a-ev 80 µl
(8 western blots)
Backorder 4 months
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GNB2L1 Antibody (N-term) - Product info

Application
  • Applications Legend:
  • WB=Western Blotting
  • IP=Immunoprecipitation
  • IHC-P=Immunohistochemistry (Paraffin)
  • IF-IC=Immunofluorescence (Immunocytochemistry)
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary AccessionP63244
Other AccessionP63245, P63246, P68040, Q4R7Y4, O42248, P63247, P63243, NP_006089
ReactivityHuman
PredictedMouse, Rat, Zebrafish, Bovine, Chicken, Pig, Monkey
IsotypeRabbit Ig
Calculated MW35077 Da

GNB2L1 Antibody (N-term) - Additional info

Gene ID 10399
Other Names
GNB2L1; Guanine nucleotide-binding protein subunit beta-2-like 1; Cell proliferation-inducing gene 21 protein; Guanine nucleotide-binding protein subunit beta-like protein 12.3; Human lung cancer oncogene 7 protein; Receptor for activated C kinase; Receptor of activated protein kinase C 1
Target/Specificity
This GNB2L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human GNB2L1.
Dilution
WB~~1:1000
IHC~~1:50~100
Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions
GNB2L1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

GNB2L1 Antibody (N-term) - Protein Information

Name GNB2L1
Function
Involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression. Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand- independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Regulates internalization of the muscarinic receptor CHRM2. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration. Binds to Y.pseudotuberculosis yopK which leads to inhibition of phagocytosis and survival of bacteria following infection of host cells. Enhances phosphorylation of HIV-1 Nef by PKCs. Promotes migration of breast carcinoma cells by binding to and activating RHOA.
Cellular Location
Cell membrane; Peripheral membrane protein. Cytoplasm. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton Nucleus. Perikaryon (By similarity). Cell projection, dendrite (By similarity). Cell projection, phagocytic cup. Note=Recruited to the plasma membrane through interaction with KRT1 which binds to membrane-bound ITGB1. Also associated with the membrane in oncogene-transformed cells. PKC activation induces translocation from the perinuclear region to the cell periphery. In the brain, detected mainly in cell bodies and dendrites with little expression in axonal fibers or nuclei. Localized to phagocytic cups following infection by Y.pestis
Tissue Location
In the liver, expressed at higher levels in activated hepatic stellate cells than in hepatocytes or Kupffer cells. Up-regulated in hepatocellular carcinomas and in the adjacent non-tumor liver tissue

GNB2L1 Antibody (N-term) - Related products

BP8063a: GNB2L1 Antibody (N-term) Blocking Peptide

GNB2L1 Antibody (N-term) - Research Areas

Application data

  • WB - GNB2L1 Antibody (N-term) AP8063a

    The anti-GNB2L1 Pab (Cat. #AP8063a) is used in Western blot to detect GNB2L1 in Ramos cell lysate.

  • WB - GNB2L1 Antibody (N-term) AP8063a

    Western blot analysis of GNB2L1 (arrow) using GNB2L1 Antibody (N-term) (Cat.#AP8063a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GNB2L1 gene (Lane 2) (Origene Technologies).

  • IHC - GNB2L1 Antibody (N-term) AP8063a

    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

  • WB - GNB2L1 Antibody (N-term) AP8063a
  • WB - GNB2L1 Antibody (N-term) AP8063a
  • IHC - GNB2L1 Antibody (N-term) AP8063a

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BACKGROUND

Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.

REFERENCES

Ceci, M., et al., Nature 426(6966):579-584 (2003).
Usacheva, A., et al., J. Immunol. 171(6):2989-2994 (2003).
Kiely, P.A., et al., J. Biol. Chem. 277(25):22581-22589 (2002).
Tcherkasowa, A.E., et al., J. Immunol. 169(9):5161-5170 (2002).
Liedtke, C.M., et al., J. Biol. Chem. 277(25):22925-22933 (2002).