|Application ||WB, IHC-P, E|
|Calculated MW||88495 Da|
|Antigen Region||323-352 aa|
|Other Names||Serine/threonine-protein kinase haspin, Germ cell-specific gene 2 protein, H-haspin, Haploid germ cell-specific nuclear protein kinase, GSG2|
|Target/Specificity||This Haspin GSG2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323~352 amino acids from the central region of human haspin.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||GSG2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine-protein kinase that phosphorylates histone H3 at 'Thr-3' (H3T3ph) during mitosis. This positions and activates AURKB and other components of the chromosomal passenger complex (CPC) at centromeres to ensure proper chromatid cohesion, metaphase alignment and normal progression through the cell cycle.|
|Cellular Location||Nucleus. Chromosome. Cytoplasm, cytoskeleton, spindle. Note=Nuclear during interphase and associates with the chromosomes and spindle apparatus during mitosis|
|Tissue Location||Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.|
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Provided below are standard protocols that you may find useful for product applications.
Post-translational modifications of conserved N-terminal tail residues in histones regulate many aspects of chromosome activity. Mitotic phosphorylation of H3 Thr 3 occurs in prophase and dephosphorylation during anaphase. Haspin, a dual serine/threonine kinase, plays an important role in regulation of chromosome and spindle function during mitosis and meiosis via its function in phosphorylation of the threonine residue in the third position of histone 3 (Thr3).
Dai, Jun et al. Genes Dev 19:472-88 (2005).
Higgins, J.M., Gene 267(1):55-69 (2001).
Tanaka, H., et al., Mol. Hum. Reprod. 7(3):211-218 (2001).
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