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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   MLKLAK Antibody (Center)   

MLKLAK Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - MLKLAK Antibody (Center) AP8068c
    Western blot analysis of anti-MLKLAK-C284 Pab (Cat. #AP8068c) in HepG2 cell line lysate (35ug/lane). MLKLAK-C284(arrow) was detected using the purified Pab. This western blot detected isoform 2 of MLKLAK, the accession number for MLKLAK isoform 1 is Q9NYL2, NP_057737.
  • IHC-P - MLKLAK Antibody (Center) AP8068c
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, E
Primary Accession Q9NYL2
Other Accession Q9ESL4, NP_598407
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 91155 Da
Antigen Region 271-300 aa
Additional Information
Gene ID 51776
Other Names Mitogen-activated protein kinase kinase kinase MLT, Human cervical cancer suppressor gene 4 protein, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase, MLK-like mitogen-activated protein triple kinase, Mixed lineage kinase-related kinase, MLK-related kinase, MRK, Sterile alpha motif- and leucine zipper-containing kinase AZK, ZAK, MLTK
Target/Specificity This MLKLAK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271-300 amino acids from the Central region of human MLKLAK.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMLKLAK Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZAK
Synonyms MLTK
Function Stress-activated component of a protein kinase signal transduction cascade. Regulates the JNK and p38 pathways. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14. Pro-apoptotic. Role in regulation of S and G2 cell cycle checkpoint by direct phosphorylation of CHEK2 (PubMed:10924358, PubMed:11836244, PubMed:15342622, PubMed:21224381). Involved in limb development (PubMed:26755636).
Cellular Location Cytoplasm. Nucleus. Note=Translocates to the nucleus upon ultraviolet B irradiation
Tissue Location Ubiquitously expressed. Isoform 2 is the predominant form in all tissues examined, except for liver, in which isoform 1 is more highly expressed
Research Areas
Citations ( 0 )

Background

MLKLAK is a member of the MAPKKK family of signal transduction molecules. It possesses an N-terminal kinase catalytic domain, followed by a leucine zipper motif and a sterile-alpha motif (SAM). This magnesium-binding protein forms homodimers and is located in the cytoplasm. The protein mediates gamma radiation signaling leading to cell cycle arrest and activity of this protein plays a role in cell cycle checkpoint regulation in cells. The protein also has pro-apoptotic activity.

References

Blume-Jensen P, et al. Nature 2001. 411: 355.
Cantrell D, J. Cell Sci. 2001. 114: 1439.
Jhiang S Oncogene 2000. 19: 5590.
Manning G, et al. Science 2002. 298: 1912.
Moller, D, et al. Am. J. Physiol. 1994. 266: C351-C359.
Robertson, S. et al. Trends Genet. 2000. 16: 368.
Robinson D, et al. Oncogene 2000. 19: 5548.
Van der Ven, P, et al. Hum. Molec. Genet. 1993. 2: 1889.
Vanhaesebroeck, B, et al. Biochem. J. 2000. 346: 561.
Van Weering D, et al. Recent Results Cancer Res. 1998. 154: 271.

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$ 295.00
$ 99.00
Cat# AP8068c
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(40 western blots)
Availability: In Stock
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