- CITATIONS: 1
|Application ||WB, E|
|Calculated MW||57705 Da|
|Antigen Region||331-361 aa|
|Other Names||Serine/threonine-protein kinase Nek3, HSPK 36, Never in mitosis A-related kinase 3, NimA-related protein kinase 3, NEK3|
|Target/Specificity||This NEK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 331-361 amino acids from the Central region of human NEK3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||NEK3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin- mediated phosphorylation of PXN and VAV2. Implicated in prolactin- mediated cytoskeletal reorganization and motility of breast cancer cells through mechanisms involving RAC1 activation and phosphorylation of PXN and VAV2.|
|Cellular Location||Cytoplasm. Cell projection, axon|
|Tissue Location||Up-regulated in malignant versus normal breast tissue. Isoform 2 shows a high level of expression in testis, ovary and brain.|
Provided below are standard protocols that you may find useful for product applications.
In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. NEK3 is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm.
Schultz, S.J., et al., Cell Growth Differ. 5(6):625-635 (1994).
Schultz, S.J., et al., Cell Growth Differ. 4(10):821-830 (1993).
Kimura, M., et al., Cytogenet. Cell Genet. 95 (3-4), 177-182 (2001).
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