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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   NEK7 Antibody (N-term)   

NEK7 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - NEK7 Antibody (N-term) AP8078a
    Western blot analysis of NEK7 (arrow) using NEK7 Antibody (N-term) (Cat.#AP8078a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NEK7 gene (Lane 2) (Origene Technologies).
  • IHC-P - NEK7 Antibody (N-term) AP8078a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • WB - NEK7 Antibody (N-term) AP8078a
    NEK7 Antibody (M8) (Cat. #AP8078a) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the NEK7 antibody detected the NEK7 protein (arrow).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q8TDX7
Other Accession D3ZBE5
Reactivity Human
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 34551 Da
Antigen Region 1-30 aa
Additional Information
Gene ID 140609
Other Names Serine/threonine-protein kinase Nek7, Never in mitosis A-related kinase 7, NimA-related protein kinase 7, NEK7
Target/Specificity This NEK7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NEK7.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsNEK7 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NEK7
Function Protein kinase which plays an important role in mitotic cell cycle progression. Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. Phosphorylates RPS6KB1.
Cellular Location Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Present at centrosome throughout the cell cycle. Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody
Tissue Location Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine
Research Areas
Citations (0)

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NIMA-related kinases share high amino acid sequence identity with the gene product of the Aspergillus nidulans 'never in mitosis A' gene, which controls initiation of mitosis.[supplied by OMIM]


Belham, C., et al., J. Biol. Chem. 278(37):34897-34909 (2003).
Kimura, M., et al., Cytogenet. Cell Genet. 94 (1-2), 33-38 (2001).

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$ 295.00
$ 99.00
Cat# AP8078a
(40 western blots)
Availability: In Stock
Bulk Size
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