|Application ||WB, IHC-P, E|
|Calculated MW||69501 Da|
|Antigen Region||544-574 aa|
|Other Names||Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2, PAPS synthase 2, PAPSS 2, Sulfurylase kinase 2, SK 2, SK2, Sulfate adenylyltransferase, ATP-sulfurylase, Sulfate adenylate transferase, SAT, Adenylyl-sulfate kinase, 3'-phosphoadenosine-5'-phosphosulfate synthase, APS kinase, Adenosine-5'-phosphosulfate 3'-phosphotransferase, Adenylylsulfate 3'-phosphotransferase, PAPSS2, ATPSK2|
|Target/Specificity||This PAPSS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 544-574 amino acids from the C-terminal region of human PAPSS2.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||PAPSS2 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Bifunctional enzyme with both ATP sulfurylase and APS kinase activity, which mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate (PAPS: activated sulfate donor used by sulfotransferase). In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate- activation pathway. May have a important role in skeletogenesis during postnatal growth (By similarity).|
|Tissue Location||Expressed in cartilage and adrenal gland.|
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Provided below are standard protocols that you may find useful for product applications.
Three-prime-phosphoadenosine 5-prime-phosphosulfate (PAPS) is the sulfate donor cosubstrate for all sulfotransferase (SULT) enzymes. SULTs catalyze the sulfate conjugation of many endogenous and exogenous compounds, including drugs and other xenobiotics. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2.
Xu, Z.H., et al., Biochem. Biophys. Res. Commun. 268(2):437-444 (2000).
Kurima, K., et al., J. Biol. Chem. 274(47):33306-33312 (1999).
ul Haque, M.F., et al., Nat. Genet. 20(2):157-162 (1998).
Kurima, K., et al., Proc. Natl. Acad. Sci. U.S.A. 95(15):8681-8685 (1998).
Shimizu, C., et al., Biochem. J. 363 (Pt 2), 263-271 (2002).
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