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PCK1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • IF - PCK1 Antibody (C-term) AP8093b
    Confocal immunofluorescent analysis of PCK1 Antibody (C-term)(Cat#AP8093b) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
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  • WB - PCK1 Antibody (C-term) AP8093b
    Western blot analysis of PCK1 Antibody (C-term) (Cat. #AP8093b) in rat primary hepatocyte cell line lysates. PCK1 (arrow) was detected using the purified Pab.
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  • WB - PCK1 Antibody (C-term) AP8093b
    The anti-PCK1 Pab (Cat. #AP8093b) is used in Western blot to detect PCK1 in mouse liver (Lane 1) and mouse small intestine tissue lysates (Lane 2).
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  • WB - PCK1 Antibody (C-term) AP8093b
    All lanes : Anti-PCK1 Antibody (C-term) at 1:2000 dilution Lane 1: Human liver lysate Lane 2: Human kidney lysate Lane 3: NCI-H460 whole cell lysate Lane 4: Mouse liver lysate Lane 5: Mouse kidney lysate Lane 6: Rat kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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  • IHC-P-Leica - PCK1 Antibody (C-term) AP8093b
    Immunohistochemical analysis of paraffin-embedded human kidney tissue using AP8093B performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/250) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
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  • SPECIFICATION
  • CITATIONS: 5
  • PROTOCOLS
  • BACKGROUND
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IF, IHC-P-Leica, E
Primary Accession P35558
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 69195 Da
Antigen Region 592-622 aa
Additional Information
Gene ID 5105
Other Names Phosphoenolpyruvate carboxykinase, cytosolic [GTP], PEPCK-C, PCK1, PEPCK1
Target/Specificity This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.
Dilution IF~~1:10~50
WB~~1:2000
IHC-P-Leica~~1:250
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPCK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PCK1 {ECO:0000303|PubMed:8490617, ECO:0000312|HGNC:HGNC:8724}
Function Cytosolic phosphoenolpyruvate carboxykinase that catalyzes the reversible decarboxylation and phosphorylation of oxaloacetate (OAA) and acts as the rate-limiting enzyme in gluconeogenesis (PubMed:30193097, PubMed:24863970, PubMed:26971250, PubMed:28216384). Regulates cataplerosis and anaplerosis, the processes that control the levels of metabolic intermediates in the citric acid cycle (PubMed:30193097, PubMed:24863970, PubMed:26971250, PubMed:28216384). At low glucose levels, it catalyzes the cataplerotic conversion of oxaloacetate to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle (PubMed:30193097). At high glucose levels, it catalyzes the anaplerotic conversion of phosphoenolpyruvate to oxaloacetate (PubMed:30193097). Acts as a regulator of formation and maintenance of memory CD8(+) T-cells: up- regulated in these cells, where it generates phosphoenolpyruvate, via gluconeogenesis (By similarity). The resultant phosphoenolpyruvate flows to glycogen and pentose phosphate pathway, which is essential for memory CD8(+) T-cells homeostasis (By similarity). In addition to the phosphoenolpyruvate carboxykinase activity, also acts as a protein kinase when phosphorylated at Ser-90: phosphorylation at Ser-90 by AKT1 reduces the binding affinity to oxaloacetate and promotes an atypical serine protein kinase activity using GTP as donor (PubMed:32322062). The protein kinase activity regulates lipogenesis: upon phosphorylation at Ser-90, translocates to the endoplasmic reticulum and catalyzes phosphorylation of INSIG proteins (INSIG1 and INSIG2), thereby disrupting the interaction between INSIG proteins and SCAP and promoting nuclear translocation of SREBP proteins (SREBF1/SREBP1 or SREBF2/SREBP2) and subsequent transcription of downstream lipogenesis- related genes (PubMed:32322062).
Cellular Location Cytoplasm, cytosol. Endoplasmic reticulum Note=Phosphorylation at Ser-90 promotes translocation to the endoplasmic reticulum.
Tissue Location Major sites of expression are liver, kidney and adipocytes.
Research Areas
Citations ( 0 )

Background

This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

References

Dunten, P., et al., J. Mol. Biol. 316(2):257-264 (2002).
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Deloukas, P., et al., Nature 414(6866):865-871 (2001).
O'Brien, R.M., et al., Biochim. Biophys. Acta 1264(3):284-288 (1995).
Ting, C.N., et al., Genomics 16(3):698-706 (1993).

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$ 365.00
$ 140.00
Cat# AP8093b
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