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APG1 (ULK1) Antibody (Center)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
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| Catalog # | Size | Availability | Price | |
| AP8104b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8104b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
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APG1 (ULK1) Antibody (Center) - Product info | |
| Application | IF, WB
|
| Primary Accession | O75385 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 112631 Da |
APG1 (ULK1) Antibody (Center) - Additional info | |
| Gene ID 8408 | |
| Other Names ULK1; KIAA0722; Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog; Unc-51-like kinase 1 | |
| Target/Specificity This APG1 (ULK1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 649~679 amino acids from the center region of human APG1. | |
| Dilution IF~~1:100 WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions APG1 (ULK1) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. | |
APG1 (ULK1) Antibody (Center) - Protein Information | |
| Name ULK1 | |
| Synonyms KIAA0722 | |
| Function Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3- kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation | |
| Cellular Location Cytoplasm, cytosol (By similarity). Preautophagosomal structure (By similarity). Note=Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome (By similarity) | |
| Tissue Location Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung | |
APG1 (ULK1) Antibody (Center) - Related products
AP8104b: APG1 (ULK1) Antibody (Center)
AP9117a: Denatured ATG1 Antibody
BP8104a: APG1 (ULK1) Antibody (N-term) Blocking Peptide
BP8104b: APG1 (ULK1) Antibody (C-term) Blocking Peptide
APG1 (ULK1) Antibody (Center) - Application data
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_(Center)_-_U251_CQ.jpg)
Fluorescent image of U251 cells stained with APG1 (ULK1) (Center) antibody. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8104b APG1 (ULK1) (Center) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). APG1 (ULK1) immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
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Western blot analysis of anti-ULK1 Pab (Cat. #AP8104b) in A2058 cell lysate. ULK1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
_(Center)_-_U251_CQ.jpg)
APG1 (ULK1) Antibody (Center) - Research Areas
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Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). Two human homologs of the yeast autophagy-specific kinase exist: ULK1(APG1) and ULK2. APG1 plays a critical role in regulating key elements of the autophagy pathway. APG1 stimulates autophagy, leading to autophagy-dependent restriction of cell growth and ultimately cell apoptosis at high levels of activity, and is a negative regulator of mTOR signaling.
REFERENCES
References for protein: 1.Scott, R., et al., Current Biology 17: 1-11 (2007). 2.Kuroyanagi, H., et al., Genomics 51(1):76-85 (1998). References for U251 cell line: 1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449]. 2. Pontén, J.,Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950]. 3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].
