- CITATIONS: 3
|Application ||WB, IHC-P, IF, E|
|Calculated MW||86564 Da|
|Other Names||Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB|
|Target/Specificity||This IKK beta antibody is generated from rabbits immunized with recombinant human IKK beta (full length sequence).|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Precautions||IKK beta Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine kinase that plays an essential role in the NF- kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF- dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation.|
|Cellular Location||Cytoplasm. Nucleus. Membrane raft. Note=Colocalized with DPP4 in membrane rafts.|
|Tissue Location||Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood|
Provided below are standard protocols that you may find useful for product applications.
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Tang, E.D., et al., J. Biol. Chem. 278(40):38566-38570 (2003).
Sakurai, H., et al., J. Biol. Chem. 278(38):36916-36923 (2003).
Ebner, K., et al., Blood 102(1):192-199 (2003).
Carter, R.S., et al., J. Biol. Chem. 278(22):19642-19648 (2003).
Huang, W.C., et al., J. Biol. Chem. 278(11):9944-9952 (2003).
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