- CITATIONS: 1
|Application ||WB, IHC-P, E|
|Calculated MW||90595 Da|
|Antigen Region||773-804 aa|
|Other Names||Diacylglycerol kinase beta, DAG kinase beta, 90 kDa diacylglycerol kinase, Diglyceride kinase beta, DGK-beta, DGKB, DAGK2, KIAA0718|
|Target/Specificity||This DGKB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 773-804 amino acids from the C-terminal region of human DGKB.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DGKB Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Exhibits high phosphorylation activity for long-chain diacylglycerols.|
Provided below are standard protocols that you may find useful for product applications.
Diacylglycerol (DAG) is an allosteric activator of protein kinase C. DAG also participates in regulating RAS and RHO family proteins by activating the guanine nucleotide exchange factors VAV and RASGRP1. DAG is also involved in the synthesis of phospholipids and triacylglycerols. Tight regulation of DAG levels is achieved via DAG kinases (DGKs), which remove DAG by phosphorylate it to phosphatidic acid. Several mammalian isozymes of DAGK have been identified
Caricasole, A., et al., J. Biol. Chem. 277(7):4790-4796 (2002).
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