|Application ||WB, IHC-P, E|
|Calculated MW||82630 Da|
|Antigen Region||598-628 aa|
|Other Names||Diacylglycerol kinase alpha, DAG kinase alpha, 80 kDa diacylglycerol kinase, Diglyceride kinase alpha, DGK-alpha, DGKA, DAGK, DAGK1|
|Target/Specificity||This DGKA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 598-628 amino acids from the C-terminal region of human DGKA.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||DGKA Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.|
|Tissue Location||Lymphocytes and oligodendroglial cells.|
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Provided below are standard protocols that you may find useful for product applications.
Upon cell stimulation, the kinase DGKA converts the second messenger diacylglycerol into phophatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity. DGKA is stimulated by calcium and phosphatidylserine, and is phosphorylated by protein kinase C. Tissue expression is in lymphocytes and oligodengroglial cells. DGKA contains 2 zinc-dependent phorbol-ester and DAG binding domains, and 2 EF-hand calcium binding domains.
Hart, T.C., et al., Genomics 22(1):246-247 (1994).
Hart, T.C., et al., Mamm. Genome 5(2):123-124 (1994).
Schaap, D., et al., FEBS Lett. 275 (1-2), 151-158 (1990).
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