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UCK Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - UCK Antibody (C-term) AP8129B
    Anti-UCK Antibody (L148) at 1:1000 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 22 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • WB - UCK Antibody (C-term) AP8129B
    The anti-UCK Pab (Cat. #AP8129b) is used in Western blot to detect UCK in HepG2 cell lysate (Lane 1) and mouse cerebellum tissue lysate (Lane 2).
  • IHC-P - UCK Antibody (C-term) AP8129B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P30085
Other Accession Q29561, Q2KIW9
Reactivity Human, Mouse
Predicted Bovine, Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 22222 Da
Antigen Region 132-162 aa
Additional Information
Gene ID 51727
Other Names UMP-CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 27414 {ECO:0000255|HAMAP-Rule:MF_03172}, Deoxycytidylate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, CK {ECO:0000255|HAMAP-Rule:MF_03172}, dCMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, Nucleoside-diphosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, 2746 {ECO:0000255|HAMAP-Rule:MF_03172}, Uridine monophosphate/cytidine monophosphate kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMP kinase {ECO:0000255|HAMAP-Rule:MF_03172}, UMP/CMPK {ECO:0000255|HAMAP-Rule:MF_03172}, CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172}
Target/Specificity This UCK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 132-162 amino acids from the C-terminal region of human UCK.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsUCK Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CMPK1 {ECO:0000255|HAMAP-Rule:MF_03172}
Function Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity.
Cellular Location Nucleus. Cytoplasm. Note=Predominantly nuclear
Tissue Location Ubiquitously expressed.
Research Areas
Citations (0)

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UCK1 (Uridine-cytidine kinase 1) phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. This enzyme does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Of note, UCK1 is able to use either ATP or GTP as a phosphate donor. UCK1 also possesses the ability to phosphorylate a number of cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.


Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Hu, R.M., et al., Proc. Natl. Acad. Sci. U.S.A. 97(17):9543-9548 (2000).
Hughes, G.J., et al., Electrophoresis 14(11):1216-1222 (1993).
Hochstrasser, D.F., et al., Electrophoresis 13(12):992-1001 (1992).

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$ 295.00
$ 99.00
Cat# AP8129B
(40 western blots)
Availability: In Stock
Bulk Size
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