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AK3 (AKL3L) Antibody (N-term)Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP8133a | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
- Protocols
- Backgrounds
AK3 (AKL3L) Antibody (N-term) - Product info | |
| Application | IHC, WB
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| Primary Accession | Q9UIJ7 |
| Other Accession | P08760 |
| Reactivity | Human, Mouse |
| Predicted | Bovine |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 25565 Da |
AK3 (AKL3L) Antibody (N-term) - Additional info | |
| Gene ID 50808 | |
| Other Names AK3; AK3L1; AK6; AKL3L; GTP:AMP phosphotransferase, mitochondrial; Adenylate kinase 3; Adenylate kinase 3 alpha-like 1 | |
| Target/Specificity This AK3 (AKL3L) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 31~61 amino acids from the N-terminal region of human AKL3L. | |
| Dilution IHC~~1:50~100 WB~~1:100~500WB~~1:1000 | |
| Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions AK3 (AKL3L) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
AK3 (AKL3L) Antibody (N-term) - Protein Information | |
| Name AK3 | |
| Synonyms AK3L1, AK6, AKL3L | |
| Cellular Location Mitochondrion matrix (By similarity). | |
AK3 (AKL3L) Antibody (N-term) - Related products
AP8133a: AK3 (AKL3L) Antibody (N-term)
AP8133b: AK3 (AKL3L) Antibody (C-term)
AK3 (AKL3L) Antibody (N-term) - Application data
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Western blot analysis of AK3 (AKL3L) antibody (N-term) (Cat.#AP8133a) in mouse heart tissue lysates (35ug/lane). AK3 (arrow) was detected using the purified Pab.
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AKL3L Antibody (G39) (Cat.#AP8133a) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the AKL3L antibody detected the AKL3L protein (arrow).
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Western blot analysis of AKL3L (arrow) using rabbit polyclonal AKL3L Antibody (G39) (Cat. #AP8133a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the AKL3L gene.
AK3 (AKL3L) Antibody (N-term) - Research Areas
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BACKGROUND
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).
REFERENCES
Noma, T., et al., Biochem. Biophys. Res. Commun. 264(3):990-997 (1999). Noma, T., et al., Biochem. J. 358 (Pt 1), 225-232 (2001).
