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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   PFKP Antibody (C-term)   

PFKP Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

  • WB - PFKP Antibody (C-term) AP8135B
    All lanes : Anti-PFKP Antibody (D769) at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • IHC-P - PFKP Antibody (C-term) AP8135B
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q01813
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85596 Da
Antigen Region 754-784 aa
Additional Information
Gene ID 5214
Other Names ATP-dependent 6-phosphofructokinase, platelet type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-P, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type C, Phosphofructo-1-kinase isozyme C, PFK-C, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKP, PFKF
Target/Specificity This PFKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-784 amino acids from the C-terminal region of human PFKP.
Dilution WB~~1:8000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPFKP Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Synonyms PFKF
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas
Citations ( 0 )


Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).


Adam, G.C., et al., Mol. Cell Proteomics 1(10):828-835 (2002).
Bonaldo, M.F., et al., Genome Res. 6(9):791-806 (1996).
Morrison, N., et al., Hum. Genet. 89(1):105-106 (1992).
Simpson, C.J., et al., Biochem. Biophys. Res. Commun. 180(1):197-203 (1991).
Nakajima, H., et al., FEBS Lett. 223(1):113-116 (1987).

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$ 295.00
$ 99.00
Cat# AP8135B
(40 western blots)
Availability: In Stock
Bulk Size
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