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>   home   >   Products   >   Primary Antibodies   >   Signal Transduction   >   Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)   

Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term) AP8137b
    Western blot analysis of PFKM polyclonal antibody (Cat. #AP8137b) in Hela cell line lysate. PFKM(arrow) was detected using the purified Pab.
  • IHC-P - Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term) AP8137b
    Formalin-fixed and paraffin-embedded human skeletal muscle reacted with PFKM Antibody (C-term)(Cat.#AP8137b), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IHC-P - Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term) AP8137b
    Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKM antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • IF - Fructose 6 Phosphate Kinase (PFKM) Antibody (C-term) AP8137b
    Confocal immunofluorescent analysis of Fructose 6 Phosphate Kinase (PFKM) Antibody(C-term)(Cat#AP8137b) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IF, E
Primary Accession P08237
Other Accession Q60HD9
Reactivity Human
Predicted Monkey
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 85183 Da
Antigen Region 746-776 aa
Additional Information
Gene ID 5213
Other Names ATP-dependent 6-phosphofructokinase, muscle type {ECO:0000255|HAMAP-Rule:MF_03184}, ATP-PFK {ECO:0000255|HAMAP-Rule:MF_03184}, PFK-M, 27111 {ECO:0000255|HAMAP-Rule:MF_03184}, 6-phosphofructokinase type A, Phosphofructo-1-kinase isozyme A, PFK-A, Phosphohexokinase {ECO:0000255|HAMAP-Rule:MF_03184}, PFKM, PFKX
Target/Specificity This Fructose 6 Phosphate Kinase (PFKM) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 746-776 amino acids from the C-terminal region of human Fructose 6 Phosphate Kinase (PFKM).
Dilution WB~~1:1000
IHC-P~~1:50~100
IF~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsFructose 6 Phosphate Kinase (PFKM) Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name PFKM
Synonyms PFKX
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.
Cellular Location Cytoplasm {ECO:0000255|HAMAP-Rule:MF_03184}.
Research Areas
Citations (0)

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Background

Phosphofructokinase catalyzes the irreversible conversion of fructose 6 phosphate to fructose 1,6 bisphosphate. Mammalian PFK is a complex isozyme consisting of 3 subunits: muscle (M), liver (L), and platelet (P). Only M type PFK isozyme is expressed in mature muscle, while erythrocytes contain both L and M subunits. Defects in PFKM are the cause of glycogen storage disease type 7 (GSD7), also known as Tarui disease.

References

Howard, T.D., et al., Genomics 34(1):122-127 (1996).
Vasconcelos, O., et al., Proc. Natl. Acad. Sci. U.S.A. 92(22):10322-10326 (1995).
Raben, N., et al., J. Biol. Chem. 268(7):4963-4967 (1993).
Yamasaki, T., et al., Gene 104(2):277-282 (1991).
Sharma, P.M., et al., J. Biol. Chem. 265(16):9006-9010 (1990).

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$ 295.00
$ 99.00
Cat# AP8137b
Size:
Quantity:
(40 western blots)
Availability: In Stock
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