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Enterokinase/Enteropeptidase Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • WB - Enterokinase/Enteropeptidase Antibody (C-term) AP8164b
    Anti-Enteropeptidase Antibody (C-term) at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 113 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, E
Primary Accession P98073
Other Accession NP_002763
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 112935 Da
Antigen Region 972-1002 aa
Additional Information
Gene ID 5651
Other Names Enteropeptidase, Enterokinase, Serine protease 7, Transmembrane protease serine 15, Enteropeptidase non-catalytic heavy chain, Enteropeptidase catalytic light chain, TMPRSS15, ENTK, PRSS7
Target/Specificity This Enterokinase/Enteropeptidase antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 972~1002 amino acids from the C-terminal region of human enteropeptidase.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsEnterokinase/Enteropeptidase Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name TMPRSS15
Synonyms ENTK, PRSS7
Function Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases.
Cellular Location Membrane; Single-pass type II membrane protein
Tissue Location Intestinal brush border.
Research Areas
Citations (0)
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Background

This enzyme converts the pancreatic proenzyme trypsinogen to trypsin, which activates other proenzymes including chymotrypsinogen and procarboxypeptidases. The precursor protein is cleaved into two chains that form a heterodimer linked by a disulfide bond. This protein is a member of the trypsin family of peptidases. Mutations in this gene cause enterokinase deficiency, a malabsorption disorder characterized by diarrhea and failure to thrive.

References

Holzinger, A., et al., Am. J. Hum. Genet. 70(1):20-25 (2002).
Kitamoto, Y., et al., Biochemistry 34(14):4562-4568 (1995).
Kitamoto, Y., et al., Proc. Natl. Acad. Sci. U.S.A. 91(16):7588-7592 (1994).
Imamura, T., et al., Am. J. Physiol. Gastrointest. Liver Physiol. 285 (6), G1235-G1241 (2003) (): ().
Freeman, T.C., et al., Clin. Chim. Acta 195 (1-2), 27-39 (1990) (): ().

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$ 182.50
$ 70.00
Cat# AP8164b
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