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BRSK1 Antibody (C-term)Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
| Country | United States
Ordering Information
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| Catalog # | Size | Availability | Price | |
| AP8168b | 0.1 mg 400 ul | In Stock | $ 255.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
| AP8168b-ev20 | 20 ug 100 ul | In Stock | $ 95.00 | DISCONTINED INQUIRE CLICK INQUIRE Add to cart |
- Specification
- Citiations : 0
- Reviews
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- Backgrounds
BRSK1 Antibody (C-term) - Product info | |
| Application | WB, IHC
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| Primary Accession | Q8TDC3 |
| Other Accession | NP_115806 |
| Reactivity | Human |
| Concentration | 0.25 mg/ml |
| Isotype | Rabbit Ig |
| Calculated MW | 85087 Da |
BRSK1 Antibody (C-term) - Additional info | |
| Gene ID 84446 | |
| Other Names BRSK1; KIAA1811; SAD1; SADB; Serine/threonine-protein kinase BRSK1; Brain-selective kinase 1; Brain-specific serine/threonine-protein kinase 1; Serine/threonine-protein kinase SAD-B; Synapses of Amphids Defective homolog 1 | |
| Target/Specificity This BRSK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human BRSK1. | |
| Dilution WB~~1:100~500WB~~1:1000 IHC~~1:10~50 | |
| Format Purified polyclonal antibody supplied in PBS with 0.10% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. | |
| Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. | |
| Precautions BRSK1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. | |
BRSK1 Antibody (C-term) - Protein Information | |
| Name BRSK1 | |
| Synonyms KIAA1811, SAD1, SADB | |
| Function Serine/threonine-protein kinase that plays a key role in polarization of neurons and centrosome duplication. Phosphorylates CDC25B, CDC25C, MAPT/TAU, RIMS1, TUBG1, TUBG2 and WEE1. Following phosphorylation and activation by STK11/LKB1, acts as a key regulator of polarization of cortical neurons, probably by mediating phosphorylation of microtubule-associated proteins such as MAPT/TAU at 'Thr-529' and 'Ser-579'. Also regulates neuron polarization by mediating phosphorylation of WEE1 at 'Ser-642' in post-mitotic neurons, leading to down-regulate WEE1 activity in polarized neurons. In neurons, localizes to synaptic vesicles and plays a role in neurotransmitter release, possibly by phosphorylating RIMS1. Also acts as a positive regulator of centrosome duplication by mediating phosphorylation of gamma- tubulin (TUBG1 and TUBG2) at 'Ser-131', leading to translocation of gamma-tubulin and its associated proteins to the centrosome Involved in the UV-induced DNA damage checkpoint response, probably by inhibiting CDK1 activity through phosphorylation and activation of WEE1, and inhibition of CDC25B and CDC25C | |
| Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, centrosome (By similarity). Cell junction, synapse (By similarity). Note=Localizes to synaptic vesicles in neurons (By similarity). Nuclear in the absence of DNA damage. Translocated to the nucleus in response to UV- or MMS-induced DNA damage | |
| Tissue Location Widely expressed, with highest levels in brain and testis. Protein levels remain constant throughout the cell cycle | |
BRSK1 Antibody (C-term) - Related products
AP8168a: SAD1 (BRSK1) Antibody (N-term)
AP8168b: BRSK1 Antibody (C-term)
BRSK1 Antibody (C-term) - Application data
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KIAA1811 Antibody (S685) (Cat. #AP8168b) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the KIAA1811 antibody detected the KIAA1811 protein (arrow).
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BRSK1 Antibody (C-term) (Cat. #AP8168b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of BRSK1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
BRSK1 Antibody (C-term) - Research Areas
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BACKGROUND
BRSK1 may be involved as a checkpoint kinase in the regulation of G2/M arrest in response to UV- or methyl methane sulfonate (MMS)-induced, but not IR-induced, DNA damage. This protein phosphorylates WEE1 and CDC25B in vitro and CDC25C in vitro and in vivo. BRSK1 is partitioned between cytoplasmic and nuclear locations in the absence of DNA damage, but translocates to the nucleus in response to Uv- or MMS-induced DNA damage. BRSK1 shares significant homology with the fission yeast Cdr2, a mitosis-regulatory kinase, and Caenorhabditis elegans SAD1, a neuronal cell polarity regulator. The BRSK1 transcript is expressed ubiquitously with the highest levels of expression in brain and testis.
REFERENCES
Lu, R., et al., J. Biol. Chem. 279(30):31164-31170 (2004). Lizcano, J.M., et al., EMBO J. 23(4):833-843 (2004).
