|Application ||WB, IHC-P, FC, E|
|Calculated MW||66574 Da|
|Other Names||Dual specificity protein phosphatase CDC14A, CDC14 cell division cycle 14 homolog A, CDC14A|
|Target/Specificity||This Cdc14 antibody is generated from rabbits immunized with a recombinant protein encoding aa 1~379 of human cdc14.|
|Format||Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||Cdc14 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. Dephosphorylates SIRT2 around early anaphase. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC-FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis.|
|Cellular Location||Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle|
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Provided below are standard protocols that you may find useful for product applications.
The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms.
Kaiser,B.K., et al. Mol. Biol. Cell 13 (7), 2289-2300 (2002)
Mailand,N., et al. Nat. Cell Biol. 4 (4), 317-322 (2002)
Bembenek,J. and Yu,H. J. Biol. Chem. 276 (51), 48237-48242 (2001)
Li,L., et al. J. Biol. Chem. 275 (4), 2410-2414 (2000)
Wong,A.K., et al. Genomics 59 (2), 248-251 (1999)
Li,L., et al. J. Biol. Chem. 272 (47), 29403-29406 (1997)
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