R MKP3 Antibody
Purified Rabbit Polyclonal Antibody (Pab)
- SPECIFICATION
- CITATIONS: 1
- PROTOCOLS
- BACKGROUND
Application
| FC, WB, E |
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Primary Accession | Q64346 |
Reactivity | Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 42319 Da |
Gene ID | 116663 |
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Other Names | Dual specificity protein phosphatase 6, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, MKP-3, Dusp6, Mkp3 |
Target/Specificity | This Rat MKP3 antibody is generated from rabbits immunized with a 6xHIS-tagged recombinant protein encoding aa 1~381 of rat MKP3. |
Dilution | WB~~1:1000 FC~~1:10~50 |
Format | Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | R MKP3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Dusp6 |
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Synonyms | Mkp3 |
Function | Inactivates MAP kinases. Has a specificity for the ERK family (PubMed:8626780). Implicated in muscle and neuronal differentiation (PubMed:8631996). Plays an important role in alleviating chronic postoperative pain. Necessary for the normal dephosphorylation of the long-lasting phosphorylated forms of spinal MAPK1/3 and MAP kinase p38 induced by peripheral surgery, which drives the resolution of acute postoperative allodynia (By similarity). Also important for dephosphorylation of MAPK1/3 in local wound tissue, which further contributes to resolution of acute pain (By similarity). |
Cellular Location | Cytoplasm. |
Tissue Location | Expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis |
Provided below are standard protocols that you may find useful for product applications.
Background
Dual specificity protein tyrosine phosphatases (dsPTPs) are a subfamily of protein tyrosine phosphatases implicated in the regulation of mitogen-activated protein kinase (MAPK). MKP-1 (also known as CL100, 3CH134, Erp, and hVH-1) exemplifies a class of dual-specificity phosphatase able to reverse the activation of MAP kinases by dephosphorylating critical tyrosine and threonine residues. MKP-3 is 36% identical to MKP-1 and it blocks both the phosphorylation and enzymatic activation of ERK2 by mitogens. MKP-3 mRNA is expressed in lung, heart, brain, and kidney, but not significantly in skeletal muscle or testis
References
Muda,M., et al. J. Biol. Chem. 271 (8), 4319-4326 (1996)
Mourey,R.J., et al. J. Biol. Chem. 271 (7), 3795-3802 (1996)
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